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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17778 | 5' | -45.3 | NC_004629.1 | + | 276468 | 0.66 | 1 |
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Target: 5'- -cUACugUUGGAGGugcgGUugGAGCCa -3' miRNA: 3'- auAUGugAAUUUCUca--CGugCUUGGc -5' |
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| 17778 | 5' | -45.3 | NC_004629.1 | + | 139766 | 0.67 | 1 |
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Target: 5'- aAUACACUUAAAGGcauuuuaGCACGuACUGu -3' miRNA: 3'- aUAUGUGAAUUUCUca-----CGUGCuUGGC- -5' |
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| 17778 | 5' | -45.3 | NC_004629.1 | + | 55004 | 0.69 | 0.999978 |
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Target: 5'- --aACACUUGgacGAGgauaaAGUGCugGAACUGu -3' miRNA: 3'- auaUGUGAAU---UUC-----UCACGugCUUGGC- -5' |
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| 17778 | 5' | -45.3 | NC_004629.1 | + | 174211 | 0.7 | 0.999878 |
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Target: 5'- aGUugAUUcuGAGGGUGCACGcucuAACCGu -3' miRNA: 3'- aUAugUGAauUUCUCACGUGC----UUGGC- -5' |
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| 17778 | 5' | -45.3 | NC_004629.1 | + | 43936 | 0.78 | 0.957592 |
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Target: 5'- --gAUACUuaaUGAAGGGUGUGCGAGCCa -3' miRNA: 3'- auaUGUGA---AUUUCUCACGUGCUUGGc -5' |
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| 17778 | 5' | -45.3 | NC_004629.1 | + | 277579 | 1.08 | 0.061977 |
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Target: 5'- gUAUACACUUAAAGAGUGCACGAACCGc -3' miRNA: 3'- -AUAUGUGAAUUUCUCACGUGCUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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