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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17964 | 5' | -54.9 | NC_004665.1 | + | 205 | 0.68 | 0.4667 |
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Target: 5'- --cCCCUAUCuGuCCCUAUCuguaAGUCCUCc -3' miRNA: 3'- aaaGGGAUAG-CuGGGGUAG----UCAGGGG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 37339 | 1.1 | 0.000507 |
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Target: 5'- gUUUCCCUAUCGACCCCAUCAGUCCCCa -3' miRNA: 3'- -AAAGGGAUAGCUGGGGUAGUCAGGGG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 233 | 1.1 | 0.000507 |
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Target: 5'- gUUUCCCUAUCGACCCCAUCAGUCCCCa -3' miRNA: 3'- -AAAGGGAUAGCUGGGGUAGUCAGGGG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 2515 | 0.74 | 0.202481 |
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Target: 5'- --aCCUUAUCGACCCCGgcaCAGUUCgCa -3' miRNA: 3'- aaaGGGAUAGCUGGGGUa--GUCAGGgG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 37294 | 0.68 | 0.487698 |
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Target: 5'- aUUCCCUcugaGUCcuGCCCCAUaauGaUCCCCa -3' miRNA: 3'- aAAGGGA----UAGc-UGGGGUAgu-C-AGGGG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 188 | 0.68 | 0.487698 |
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Target: 5'- aUUCCCUcugaGUCcuGCCCCAUaauGaUCCCCa -3' miRNA: 3'- aAAGGGA----UAGc-UGGGGUAgu-C-AGGGG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 16666 | 0.67 | 0.530949 |
|
Target: 5'- -cUCCCUca-GACgCUCAcgaaagaccgcUCGGUCCCCg -3' miRNA: 3'- aaAGGGAuagCUG-GGGU-----------AGUCAGGGG- -5' |
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| 17964 | 5' | -54.9 | NC_004665.1 | + | 16499 | 0.66 | 0.586802 |
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Target: 5'- -gUCCUUcUUGACCUUcagagaGUCGGUCCCa -3' miRNA: 3'- aaAGGGAuAGCUGGGG------UAGUCAGGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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