Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17971 | 5' | -55.6 | NC_004665.1 | + | 7543 | 0.66 | 0.590721 |
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Target: 5'- aGCGgCAcGACCgccgUUCUC-CCACGCUUc -3' miRNA: 3'- gUGUgGU-CUGGa---AGGAGuGGUGCGAG- -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 6171 | 0.66 | 0.579434 |
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Target: 5'- aUAgACCucgaaGCCUUCCUCACgGagaaGCUCa -3' miRNA: 3'- -GUgUGGuc---UGGAAGGAGUGgUg---CGAG- -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 1716 | 0.66 | 0.53374 |
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Target: 5'- uGCGCCAGACUggUCC-CACUcgguccaAgGCUCg -3' miRNA: 3'- gUGUGGUCUGGa-AGGaGUGG-------UgCGAG- -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 29379 | 0.67 | 0.523872 |
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Target: 5'- aGCuCCcauaAGACagguugUCCUCACCACGCa- -3' miRNA: 3'- gUGuGG----UCUGga----AGGAGUGGUGCGag -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 22005 | 0.69 | 0.391295 |
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Target: 5'- uCGCACC-GACCUgcgCCUCAUCACuGUg- -3' miRNA: 3'- -GUGUGGuCUGGAa--GGAGUGGUG-CGag -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 21322 | 0.7 | 0.324597 |
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Target: 5'- aGCACUgcGCCUUCCUCaauguugaacguccaGCCACGCg- -3' miRNA: 3'- gUGUGGucUGGAAGGAG---------------UGGUGCGag -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 19812 | 0.71 | 0.305368 |
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Target: 5'- -uUACCAagauGACgCUUCCU-ACCGCGCUCa -3' miRNA: 3'- guGUGGU----CUG-GAAGGAgUGGUGCGAG- -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 23900 | 0.71 | 0.297618 |
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Target: 5'- gGCACCAGAU--UCCUCguaguucaGCCACGCa- -3' miRNA: 3'- gUGUGGUCUGgaAGGAG--------UGGUGCGag -5' |
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| 17971 | 5' | -55.6 | NC_004665.1 | + | 5275 | 1.1 | 0.000404 |
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Target: 5'- aCACACCAGACCUUCCUCACCACGCUCa -3' miRNA: 3'- -GUGUGGUCUGGAAGGAGUGGUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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