miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17972 3' -57.3 NC_004665.1 + 34673 0.66 0.454576
Target:  5'- ------aGCCCGAuauuGCGACUGCGCagaUCAg -3'
miRNA:   3'- acagaaaCGGGCU----CGCUGGCGCG---AGU- -5'
17972 3' -57.3 NC_004665.1 + 19842 0.68 0.360325
Target:  5'- aGUCgagGCCCGAGUGGgCGC-UUCGa -3'
miRNA:   3'- aCAGaaaCGGGCUCGCUgGCGcGAGU- -5'
17972 3' -57.3 NC_004665.1 + 11044 0.69 0.302934
Target:  5'- gGUCgagGCCgaagguugcCGGGUGAUCGUGCUCGa -3'
miRNA:   3'- aCAGaaaCGG---------GCUCGCUGGCGCGAGU- -5'
17972 3' -57.3 NC_004665.1 + 28872 0.7 0.287883
Target:  5'- uUGUCUUgaaGCCUuccugcuuGUGGCUGCGCUCAc -3'
miRNA:   3'- -ACAGAAa--CGGGcu------CGCUGGCGCGAGU- -5'
17972 3' -57.3 NC_004665.1 + 26969 0.73 0.178024
Target:  5'- cGUCguagagaGUCCGAGCGAUC-CGCUCAa -3'
miRNA:   3'- aCAGaaa----CGGGCUCGCUGGcGCGAGU- -5'
17972 3' -57.3 NC_004665.1 + 6131 1.09 0.000377
Target:  5'- gUGUCUUUGCCCGAGCGACCGCGCUCAg -3'
miRNA:   3'- -ACAGAAACGGGCUCGCUGGCGCGAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.