Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17972 | 5' | -50.8 | NC_004665.1 | + | 16570 | 0.66 | 0.829897 |
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Target: 5'- aGUGAUaACCCaauGGCgUCaAUGCGggGACc -3' miRNA: 3'- cUAUUAgUGGG---CCG-AG-UACGCuuCUG- -5' |
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| 17972 | 5' | -50.8 | NC_004665.1 | + | 4958 | 0.66 | 0.819125 |
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Target: 5'- ----uUgGCaCCGGCUCAUccuuccgGCGAAGAUc -3' miRNA: 3'- cuauuAgUG-GGCCGAGUA-------CGCUUCUG- -5' |
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| 17972 | 5' | -50.8 | NC_004665.1 | + | 2090 | 0.67 | 0.746019 |
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Target: 5'- -uUGAUCGCCCacgguguuGCUUGUGCGAGguuGACg -3' miRNA: 3'- cuAUUAGUGGGc-------CGAGUACGCUU---CUG- -5' |
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| 17972 | 5' | -50.8 | NC_004665.1 | + | 26684 | 0.68 | 0.734791 |
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Target: 5'- ---uGUCACCCGGCUCAccaGAGcGCa -3' miRNA: 3'- cuauUAGUGGGCCGAGUacgCUUcUG- -5' |
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| 17972 | 5' | -50.8 | NC_004665.1 | + | 2200 | 0.69 | 0.66543 |
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Target: 5'- cGAU-GUCACCCGGCUUGaucgGC-AGGGCu -3' miRNA: 3'- -CUAuUAGUGGGCCGAGUa---CGcUUCUG- -5' |
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| 17972 | 5' | -50.8 | NC_004665.1 | + | 6094 | 1.1 | 0.001432 |
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Target: 5'- cGAUAAUCACCCGGCUCAUGCGAAGACc -3' miRNA: 3'- -CUAUUAGUGGGCCGAGUACGCUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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