Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17974 | 3' | -57.3 | NC_004665.1 | + | 2784 | 0.66 | 0.474115 |
Target: 5'- gGAGCAGGuUCACa-GCACGaCCACCAc -3' miRNA: 3'- -CUUGUCC-AGUGccCGUGUcGGUGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 26230 | 0.66 | 0.474115 |
Target: 5'- -cGCAGGUCuuGGaGCAU--CCGCCAGc -3' miRNA: 3'- cuUGUCCAGugCC-CGUGucGGUGGUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 25133 | 0.66 | 0.473081 |
Target: 5'- aGGGCAGcUCgGCGGGUgguucuaACGGCaCACCAGc -3' miRNA: 3'- -CUUGUCcAG-UGCCCG-------UGUCG-GUGGUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 23648 | 0.66 | 0.453638 |
Target: 5'- cGAACacgAGG-CGCGGGaCugGGCgACCAa -3' miRNA: 3'- -CUUG---UCCaGUGCCC-GugUCGgUGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 6689 | 0.67 | 0.414185 |
Target: 5'- cAGCAuGUCGCuGGCAUAGCC-CCAu -3' miRNA: 3'- cUUGUcCAGUGcCCGUGUCGGuGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 26743 | 0.67 | 0.395265 |
Target: 5'- cGAACcgauGGUCGCuGGguCAGCCGCaUAGg -3' miRNA: 3'- -CUUGu---CCAGUGcCCguGUCGGUG-GUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 14898 | 0.69 | 0.317334 |
Target: 5'- ---aGGGUCACGaGGUACucauucaucccGGCCACCGu -3' miRNA: 3'- cuugUCCAGUGC-CCGUG-----------UCGGUGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 30002 | 0.69 | 0.289568 |
Target: 5'- cGAACAGGUUACGGGCuucgAggaaguuguuguucuCAGCaccCACCAGc -3' miRNA: 3'- -CUUGUCCAGUGCCCG----U---------------GUCG---GUGGUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 31096 | 0.7 | 0.279296 |
Target: 5'- -uGCAGGcUCA-GGGUAU-GCCACCAGa -3' miRNA: 3'- cuUGUCC-AGUgCCCGUGuCGGUGGUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 25847 | 0.7 | 0.272138 |
Target: 5'- cGGugAGGUCAucuCGGGCuacuucaacaACGGCUugCAGa -3' miRNA: 3'- -CUugUCCAGU---GCCCG----------UGUCGGugGUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 28514 | 0.72 | 0.197416 |
Target: 5'- cGAGCAGGcCACuGGCAUcgaaGGCCACUAu -3' miRNA: 3'- -CUUGUCCaGUGcCCGUG----UCGGUGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 20661 | 0.73 | 0.173874 |
Target: 5'- cGGACAGGaucgcgcuguagucuUCugGGGCGCAGUagaagcgacgauCACCAGc -3' miRNA: 3'- -CUUGUCC---------------AGugCCCGUGUCG------------GUGGUC- -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 32087 | 0.74 | 0.141234 |
Target: 5'- -uGCGGG-CACGGGCAUcaaGGUCACCAu -3' miRNA: 3'- cuUGUCCaGUGCCCGUG---UCGGUGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 2589 | 0.75 | 0.122459 |
Target: 5'- uGAGCGGGUCGUGGGCACAGCgaagaAUCAu -3' miRNA: 3'- -CUUGUCCAGUGCCCGUGUCGg----UGGUc -5' |
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17974 | 3' | -57.3 | NC_004665.1 | + | 9059 | 1.08 | 0.000424 |
Target: 5'- aGAACAGGUCACGGGCACAGCCACCAGc -3' miRNA: 3'- -CUUGUCCAGUGCCCGUGUCGGUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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