miRNA: 3'- caUGCcUUUUCGGUGCGacgCUGGCA- -5'
miRNA: 3'- caUGCcUUUUCGGuGCGACGCU---GGCA- -5'
miRNA: 3'- caUGCCUUUUC-GGugCgaCGCUGGca -5'
miRNA: 3'- cauGCCUUuu------CGGUGCGa----CGCUGGCA- -5'
miRNA: 3'- -CAUGCCUUUUCGGUGCGACGCUGGCA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17976 | 5' | -54.2 | NC_004665.1 | + | 4586 | 0.66 | 0.639547 |
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Target: 5'- -aACGaGAAAGCCAUGCa--GGCCGUg -3' miRNA: 3'- caUGCcUUUUCGGUGCGacgCUGGCA- -5' |
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| 17976 | 5' | -54.2 | NC_004665.1 | + | 31119 | 0.66 | 0.62809 |
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Target: 5'- -cACGaAGAAGCC-CGUUGCGAggcCCGUu -3' miRNA: 3'- caUGCcUUUUCGGuGCGACGCU---GGCA- -5' |
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| 17976 | 5' | -54.2 | NC_004665.1 | + | 10098 | 0.66 | 0.616636 |
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Target: 5'- -aGCGGGAAGGuCUugGaaGCGGCCu- -3' miRNA: 3'- caUGCCUUUUC-GGugCgaCGCUGGca -5' |
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| 17976 | 5' | -54.2 | NC_004665.1 | + | 31046 | 0.7 | 0.431422 |
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Target: 5'- --gUGGAGucguagauGCCACGCaugagGCGACCGUu -3' miRNA: 3'- cauGCCUUuu------CGGUGCGa----CGCUGGCA- -5' |
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| 17976 | 5' | -54.2 | NC_004665.1 | + | 9823 | 1.07 | 0.001112 |
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Target: 5'- gGUACGGAAAAGCCACGCUGCGACCGUc -3' miRNA: 3'- -CAUGCCUUUUCGGUGCGACGCUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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