Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17977 | 3' | -58.8 | NC_004665.1 | + | 28355 | 0.66 | 0.366946 |
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Target: 5'- uUCGCCaAGGCGAUuGCCUCugGg-- -3' miRNA: 3'- gAGUGGcUCCGCUAuCGGGGugCgaa -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 24905 | 0.66 | 0.366946 |
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Target: 5'- aCUCACCGAuagcGGUgcgGAUGGuCCCUugGUUUc -3' miRNA: 3'- -GAGUGGCU----CCG---CUAUC-GGGGugCGAA- -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 14581 | 0.67 | 0.357348 |
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Target: 5'- aUCGCUGAgcugaagGGCGAgaccaAGUUCCGCGCUg -3' miRNA: 3'- gAGUGGCU-------CCGCUa----UCGGGGUGCGAa -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 1234 | 0.67 | 0.332898 |
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Target: 5'- uUCGCCGAgGGUGuaAGCCUCAUGUUc -3' miRNA: 3'- gAGUGGCU-CCGCuaUCGGGGUGCGAa -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 13316 | 0.68 | 0.286283 |
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Target: 5'- aCUCgACCGGGGUGuaugguGCCCCagcgaaggucucGCGCUUg -3' miRNA: 3'- -GAG-UGGCUCCGCuau---CGGGG------------UGCGAA- -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 32764 | 0.69 | 0.258162 |
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Target: 5'- gUCACCGGGGCc---GCCaCCAUGCa- -3' miRNA: 3'- gAGUGGCUCCGcuauCGG-GGUGCGaa -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 20242 | 0.69 | 0.258162 |
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Target: 5'- uCUCACCcggagcGAGGUaGUAGCCCUugGUg- -3' miRNA: 3'- -GAGUGG------CUCCGcUAUCGGGGugCGaa -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 18752 | 0.69 | 0.232339 |
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Target: 5'- gCUCACCGAGGcCGAgaaGGCUCaaGCGCa- -3' miRNA: 3'- -GAGUGGCUCC-GCUa--UCGGGg-UGCGaa -5' |
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| 17977 | 3' | -58.8 | NC_004665.1 | + | 12767 | 1.04 | 0.000517 |
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Target: 5'- uCUCACCGAGGCGAUAGCCCCACGCUUc -3' miRNA: 3'- -GAGUGGCUCCGCUAUCGGGGUGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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