Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17981 | 3' | -51.1 | NC_004665.1 | + | 4653 | 0.66 | 0.8458 |
Target: 5'- -uUCG-CCUCAaccuguGCGUCUcuggUCGcgCUCCa -3' miRNA: 3'- guAGCuGGAGU------UGCGGA----AGCuaGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 5141 | 0.67 | 0.794666 |
Target: 5'- cCAUCGAguagaccucauaggUCUCAGCGacgauCCagUCGAUCUCUg -3' miRNA: 3'- -GUAGCU--------------GGAGUUGC-----GGa-AGCUAGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 5393 | 0.66 | 0.85472 |
Target: 5'- aGUCgGGCCauguggUCAACGCCUgcaaGAUCagCCg -3' miRNA: 3'- gUAG-CUGG------AGUUGCGGAag--CUAGa-GG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 5450 | 0.67 | 0.77707 |
Target: 5'- gCGUUGACCaCAugGCC--CGA-CUCCa -3' miRNA: 3'- -GUAGCUGGaGUugCGGaaGCUaGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 9234 | 0.7 | 0.619923 |
Target: 5'- aCGUCGAUgUUCGAU-CCUUCGAUCUuCCa -3' miRNA: 3'- -GUAGCUG-GAGUUGcGGAAGCUAGA-GG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 9284 | 0.66 | 0.85472 |
Target: 5'- aGUCGaACUUCuggauCGCCUcggagacacaaUCGGUCUCg -3' miRNA: 3'- gUAGC-UGGAGuu---GCGGA-----------AGCUAGAGg -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 10351 | 0.68 | 0.755762 |
Target: 5'- aGUCGAUCUCA---CCUUCGGUgaCCa -3' miRNA: 3'- gUAGCUGGAGUugcGGAAGCUAgaGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 10941 | 0.72 | 0.506819 |
Target: 5'- -uUCGACC---GCGCCUUCGAugaaaUCUUCg -3' miRNA: 3'- guAGCUGGaguUGCGGAAGCU-----AGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 11747 | 0.7 | 0.643002 |
Target: 5'- aGUCGGCCUguACGCgCUUgcucaCGGUgUCCu -3' miRNA: 3'- gUAGCUGGAguUGCG-GAA-----GCUAgAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 12075 | 0.68 | 0.766492 |
Target: 5'- gCGUCaGCUUCAcccuugagguAUGCCUUCGAggUUUCCu -3' miRNA: 3'- -GUAGcUGGAGU----------UGCGGAAGCU--AGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 12988 | 0.67 | 0.817604 |
Target: 5'- -uUCGACCUCGAaggUGCUgagCGA-CUCUa -3' miRNA: 3'- guAGCUGGAGUU---GCGGaa-GCUaGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 13594 | 0.67 | 0.817604 |
Target: 5'- -cUCGACCaguacCAGCGCCguucgggUUGAUCgaaCCg -3' miRNA: 3'- guAGCUGGa----GUUGCGGa------AGCUAGa--GG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 16132 | 0.67 | 0.787482 |
Target: 5'- -cUCGcuguacGCCUCAACGCCUcguaccUGAUCgCCa -3' miRNA: 3'- guAGC------UGGAGUUGCGGAa-----GCUAGaGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 18059 | 1.12 | 0.001102 |
Target: 5'- cCAUCGACCUCAACGCCUUCGAUCUCCu -3' miRNA: 3'- -GUAGCUGGAGUUGCGGAAGCUAGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 19230 | 0.69 | 0.700297 |
Target: 5'- --gUGACCUCGACa---UCGGUCUCCu -3' miRNA: 3'- guaGCUGGAGUUGcggaAGCUAGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 20300 | 0.66 | 0.85472 |
Target: 5'- cCGUCGAUCUgGAUGaCCUUCucggaaugcuuGAUgUCCu -3' miRNA: 3'- -GUAGCUGGAgUUGC-GGAAG-----------CUAgAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 25040 | 0.66 | 0.8458 |
Target: 5'- uCAUCaaGACCUCGG-GCCUgUCGAacgCUCUg -3' miRNA: 3'- -GUAG--CUGGAGUUgCGGA-AGCUa--GAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 29164 | 0.69 | 0.700297 |
Target: 5'- --cCGAUCUCAGCgGCCagcuugUUGGUCUUCg -3' miRNA: 3'- guaGCUGGAGUUG-CGGa-----AGCUAGAGG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 32575 | 0.68 | 0.722794 |
Target: 5'- uGUCGGCCUCAGucuuCGC--UCGGUCggCCu -3' miRNA: 3'- gUAGCUGGAGUU----GCGgaAGCUAGa-GG- -5' |
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17981 | 3' | -51.1 | NC_004665.1 | + | 33852 | 0.66 | 0.836635 |
Target: 5'- cCAUUGACCUCGcuaagaaucuGCGCgUUCaGAUCa-- -3' miRNA: 3'- -GUAGCUGGAGU----------UGCGgAAG-CUAGagg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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