Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17983 | 5' | -53.8 | NC_004665.1 | + | 31874 | 0.66 | 0.692731 |
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Target: 5'- cGCCgacugACCuCUGCaacUGAGCGUCUggUGGa -3' miRNA: 3'- -CGG-----UGG-GACGa--ACUCGUAGAgaACCc -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 25886 | 0.67 | 0.589836 |
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Target: 5'- aCCGCCCgaagGCgaGuGCAUCacaUUGGGa -3' miRNA: 3'- cGGUGGGa---CGaaCuCGUAGag-AACCC- -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 27163 | 0.68 | 0.555847 |
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Target: 5'- aCCGCCCUGCcacGAGUuaacGUCUgcUGGGu -3' miRNA: 3'- cGGUGGGACGaa-CUCG----UAGAgaACCC- -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 91 | 0.69 | 0.479202 |
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Target: 5'- cGCCucgguuCCCU-CaaGGGCAUCUCaUGGGg -3' miRNA: 3'- -CGGu-----GGGAcGaaCUCGUAGAGaACCC- -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 37197 | 0.69 | 0.479202 |
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Target: 5'- cGCCucgguuCCCU-CaaGGGCAUCUCaUGGGg -3' miRNA: 3'- -CGGu-----GGGAcGaaCUCGUAGAGaACCC- -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 13376 | 0.72 | 0.3188 |
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Target: 5'- aGCCAgCCUuCUUGAGCAUCUUgaugaugUGaGGg -3' miRNA: 3'- -CGGUgGGAcGAACUCGUAGAGa------AC-CC- -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 10605 | 0.72 | 0.3188 |
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Target: 5'- cGUUGCCCUGCUUGAcGCA-CUCgUUGGc -3' miRNA: 3'- -CGGUGGGACGAACU-CGUaGAG-AACCc -5' |
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| 17983 | 5' | -53.8 | NC_004665.1 | + | 18846 | 1.13 | 0.000414 |
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Target: 5'- gGCCACCCUGCUUGAGCAUCUCUUGGGa -3' miRNA: 3'- -CGGUGGGACGAACUCGUAGAGAACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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