Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17984 | 3' | -52.5 | NC_004665.1 | + | 27493 | 0.66 | 0.778758 |
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Target: 5'- gGguGCCUUCugcuaccagcuuGGCGUAUUCGUCu--- -3' miRNA: 3'- aCuuCGGAAG------------CCGCAUGAGCAGcuag -5' |
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| 17984 | 3' | -52.5 | NC_004665.1 | + | 21465 | 0.66 | 0.757516 |
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Target: 5'- gUGAuGUCgUUGGUGUAaaCGUCGGUCu -3' miRNA: 3'- -ACUuCGGaAGCCGCAUgaGCAGCUAG- -5' |
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| 17984 | 3' | -52.5 | NC_004665.1 | + | 31924 | 0.66 | 0.746677 |
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Target: 5'- aGAGGUCagUCGGCGaauCUCGaUCaGGUCg -3' miRNA: 3'- aCUUCGGa-AGCCGCau-GAGC-AG-CUAG- -5' |
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| 17984 | 3' | -52.5 | NC_004665.1 | + | 24067 | 0.67 | 0.702178 |
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Target: 5'- uUGGAGCUggugaUGGUG-ACUCGUUGGUUg -3' miRNA: 3'- -ACUUCGGaa---GCCGCaUGAGCAGCUAG- -5' |
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| 17984 | 3' | -52.5 | NC_004665.1 | + | 7490 | 0.68 | 0.610376 |
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Target: 5'- cGuAAGCCUU-GGUGauCUUGUCGAUCa -3' miRNA: 3'- aC-UUCGGAAgCCGCauGAGCAGCUAG- -5' |
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| 17984 | 3' | -52.5 | NC_004665.1 | + | 19365 | 1.1 | 0.001046 |
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Target: 5'- cUGAAGCCUUCGGCGUACUCGUCGAUCu -3' miRNA: 3'- -ACUUCGGAAGCCGCAUGAGCAGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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