Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17984 | 5' | -53.5 | NC_004665.1 | + | 20705 | 0.66 | 0.672925 |
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Target: 5'- uGGG-UCGAUCAGCgcagcguaguucgCAGCGUUcGGCa -3' miRNA: 3'- -CCUaAGCUGGUCGa------------GUCGUAGuCCGa -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 20531 | 0.67 | 0.643002 |
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Target: 5'- ---aUCGACCAGaUCAGCAgc-GGCa -3' miRNA: 3'- ccuaAGCUGGUCgAGUCGUaguCCGa -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 6585 | 0.67 | 0.643002 |
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Target: 5'- cGAUU-GGCCAGC-CAcGgAUCAGGCg -3' miRNA: 3'- cCUAAgCUGGUCGaGU-CgUAGUCCGa -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 21256 | 0.67 | 0.643002 |
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Target: 5'- aGGAUgCGGCgAGCguucgccacgUCAGCGUUAGGg- -3' miRNA: 3'- -CCUAaGCUGgUCG----------AGUCGUAGUCCga -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 7933 | 0.67 | 0.643002 |
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Target: 5'- aGAcUCGaACCAGUaCGGC-UCGGGCg -3' miRNA: 3'- cCUaAGC-UGGUCGaGUCGuAGUCCGa -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 36255 | 0.67 | 0.608395 |
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Target: 5'- uGGA--UGACCAGCUuguggcugccCAGUA-CAGGCUc -3' miRNA: 3'- -CCUaaGCUGGUCGA----------GUCGUaGUCCGA- -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 13611 | 0.67 | 0.607243 |
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Target: 5'- cGGGUaggcgugugucgcUCGACCAGUacCAGCGccguUCGGGUUg -3' miRNA: 3'- -CCUA-------------AGCUGGUCGa-GUCGU----AGUCCGA- -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 22358 | 0.68 | 0.573989 |
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Target: 5'- -----gGGCCAGCUCAGCGgcaauccaUgAGGCa -3' miRNA: 3'- ccuaagCUGGUCGAGUCGU--------AgUCCGa -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 21043 | 0.69 | 0.474488 |
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Target: 5'- gGGGUgaaGACCAGCgcacCAGCAgcuUCAGGaCg -3' miRNA: 3'- -CCUAag-CUGGUCGa---GUCGU---AGUCC-Ga -5' |
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| 17984 | 5' | -53.5 | NC_004665.1 | + | 19331 | 1.09 | 0.000781 |
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Target: 5'- uGGAUUCGACCAGCUCAGCAUCAGGCUc -3' miRNA: 3'- -CCUAAGCUGGUCGAGUCGUAGUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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