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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17985 | 5' | -56.8 | NC_004665.1 | + | 14400 | 0.66 | 0.487698 |
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Target: 5'- -cUGuuGGGCCAcCUUAGCGaCGAUCu -3' miRNA: 3'- caACugCCCGGUcGAGUCGCcGUUAG- -5' |
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| 17985 | 5' | -56.8 | NC_004665.1 | + | 25129 | 0.67 | 0.38781 |
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Target: 5'- -aUGAaGGG-CAGCUCGGCGGguGg- -3' miRNA: 3'- caACUgCCCgGUCGAGUCGCCguUag -5' |
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| 17985 | 5' | -56.8 | NC_004665.1 | + | 5232 | 0.69 | 0.310196 |
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Target: 5'- --aGGUGGGUCAgGCUCAGCGGUggcugaagGGUCa -3' miRNA: 3'- caaCUGCCCGGU-CGAGUCGCCG--------UUAG- -5' |
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| 17985 | 5' | -56.8 | NC_004665.1 | + | 26855 | 0.71 | 0.218063 |
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Target: 5'- --cGAUGcucauguucuugguGGCCuuCUCAGCGGCAAUCa -3' miRNA: 3'- caaCUGC--------------CCGGucGAGUCGCCGUUAG- -5' |
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| 17985 | 5' | -56.8 | NC_004665.1 | + | 7570 | 0.75 | 0.114662 |
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Target: 5'- cUUGccACGGGCCAGCUuggcuugagCAGCGGCAc-- -3' miRNA: 3'- cAAC--UGCCCGGUCGA---------GUCGCCGUuag -5' |
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| 17985 | 5' | -56.8 | NC_004665.1 | + | 22365 | 1.08 | 0.000372 |
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Target: 5'- aGUUGACGGGCCAGCUCAGCGGCAAUCc -3' miRNA: 3'- -CAACUGCCCGGUCGAGUCGCCGUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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