Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17986 | 3' | -56 | NC_004665.1 | + | 17630 | 0.66 | 0.541986 |
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Target: 5'- aCGAGCUGCUUUgCcUGcCACUCGGAg -3' miRNA: 3'- gGCUCGACGGGAaGuACcGUGAGCUU- -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 28221 | 0.66 | 0.540879 |
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Target: 5'- aCCGGGCUucacccaGUCCaacgggUCAUGGUcCUCGAc -3' miRNA: 3'- -GGCUCGA-------CGGGa-----AGUACCGuGAGCUu -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 28323 | 0.66 | 0.530949 |
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Target: 5'- aCG-GCUGCCCUUgGU-GUAgUCGAAg -3' miRNA: 3'- gGCuCGACGGGAAgUAcCGUgAGCUU- -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 30659 | 0.66 | 0.509131 |
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Target: 5'- uCCGAGCUGCUCaugg-GGaCACUcCGAGa -3' miRNA: 3'- -GGCUCGACGGGaaguaCC-GUGA-GCUU- -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 30202 | 0.66 | 0.509131 |
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Target: 5'- -aGAGCuguUGCCCUUgGUGGU-CUUGAGc -3' miRNA: 3'- ggCUCG---ACGGGAAgUACCGuGAGCUU- -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 33938 | 0.67 | 0.44618 |
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Target: 5'- gCGAGCUGCCCcuaUCGccgcuggagcUGGUGC-CGAAg -3' miRNA: 3'- gGCUCGACGGGa--AGU----------ACCGUGaGCUU- -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 2794 | 0.68 | 0.426172 |
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Target: 5'- uUGAGaucGCCCUUCAgUGGgACUUGAAg -3' miRNA: 3'- gGCUCga-CGGGAAGU-ACCgUGAGCUU- -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 28708 | 0.69 | 0.35179 |
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Target: 5'- uUGAGCUGCCUgaaggCAUGGCACc---- -3' miRNA: 3'- gGCUCGACGGGaa---GUACCGUGagcuu -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 7557 | 0.71 | 0.250959 |
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Target: 5'- gCGAGCUGCCUUUCuucgagaacgaggAUGGCAC-CGu- -3' miRNA: 3'- gGCUCGACGGGAAG-------------UACCGUGaGCuu -5' |
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| 17986 | 3' | -56 | NC_004665.1 | + | 25182 | 1.08 | 0.000523 |
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Target: 5'- gCCGAGCUGCCCUUCAUGGCACUCGAAa -3' miRNA: 3'- -GGCUCGACGGGAAGUACCGUGAGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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