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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17986 | 5' | -54.3 | NC_004665.1 | + | 34808 | 0.68 | 0.552333 |
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Target: 5'- aCUUGGGCGUcuGCUCGcugGGCuuGCUCGAu -3' miRNA: 3'- gGAACCUGCA--UGGGUaa-CCG--UGGGCU- -5' |
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| 17986 | 5' | -54.3 | NC_004665.1 | + | 1358 | 0.68 | 0.541313 |
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Target: 5'- cCCUUgGGGCGUuccaacagaugaACCCAgagacgaUGGCAUCCa- -3' miRNA: 3'- -GGAA-CCUGCA------------UGGGUa------ACCGUGGGcu -5' |
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| 17986 | 5' | -54.3 | NC_004665.1 | + | 10612 | 0.71 | 0.382819 |
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Target: 5'- gCCUUGG-CGUugcccugcuugacgcACUCGUUGGCAUCCu- -3' miRNA: 3'- -GGAACCuGCA---------------UGGGUAACCGUGGGcu -5' |
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| 17986 | 5' | -54.3 | NC_004665.1 | + | 24748 | 0.72 | 0.352554 |
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Target: 5'- gCgUUGGGCGUcCUCAgcGGCACCCu- -3' miRNA: 3'- -GgAACCUGCAuGGGUaaCCGUGGGcu -5' |
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| 17986 | 5' | -54.3 | NC_004665.1 | + | 25147 | 1.11 | 0.000644 |
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Target: 5'- cCCUUGGACGUACCCAUUGGCACCCGAc -3' miRNA: 3'- -GGAACCUGCAUGGGUAACCGUGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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