miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
17986 5' -54.3 NC_004665.1 + 34808 0.68 0.552333
Target:  5'- aCUUGGGCGUcuGCUCGcugGGCuuGCUCGAu -3'
miRNA:   3'- gGAACCUGCA--UGGGUaa-CCG--UGGGCU- -5'
17986 5' -54.3 NC_004665.1 + 1358 0.68 0.541313
Target:  5'- cCCUUgGGGCGUuccaacagaugaACCCAgagacgaUGGCAUCCa- -3'
miRNA:   3'- -GGAA-CCUGCA------------UGGGUa------ACCGUGGGcu -5'
17986 5' -54.3 NC_004665.1 + 10612 0.71 0.382819
Target:  5'- gCCUUGG-CGUugcccugcuugacgcACUCGUUGGCAUCCu- -3'
miRNA:   3'- -GGAACCuGCA---------------UGGGUAACCGUGGGcu -5'
17986 5' -54.3 NC_004665.1 + 24748 0.72 0.352554
Target:  5'- gCgUUGGGCGUcCUCAgcGGCACCCu- -3'
miRNA:   3'- -GgAACCUGCAuGGGUaaCCGUGGGcu -5'
17986 5' -54.3 NC_004665.1 + 25147 1.11 0.000644
Target:  5'- cCCUUGGACGUACCCAUUGGCACCCGAc -3'
miRNA:   3'- -GGAACCUGCAUGGGUAACCGUGGGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.