Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17989 | 3' | -56.2 | NC_004665.1 | + | 24449 | 0.66 | 0.535501 |
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Target: 5'- aAG-CGCAuCCGUuGUCCCUugGUCAg -3' miRNA: 3'- -UCaGCGUcGGUAcUAGGGGugCAGUg -5' |
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| 17989 | 3' | -56.2 | NC_004665.1 | + | 4279 | 0.66 | 0.534405 |
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Target: 5'- uGUCGUAGCCAgcucgggugguggUGAcagCCUCACcgaugGUCACu -3' miRNA: 3'- uCAGCGUCGGU-------------ACUa--GGGGUG-----CAGUG- -5' |
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| 17989 | 3' | -56.2 | NC_004665.1 | + | 14920 | 0.66 | 0.524574 |
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Target: 5'- ---gGCAGCCuucuUGAgUCCCCAgaggGUCACg -3' miRNA: 3'- ucagCGUCGGu---ACU-AGGGGUg---CAGUG- -5' |
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| 17989 | 3' | -56.2 | NC_004665.1 | + | 17251 | 0.67 | 0.471359 |
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Target: 5'- -uUCGCAGCUucgGUGucggaaucGUCCUCACuGUCACu -3' miRNA: 3'- ucAGCGUCGG---UAC--------UAGGGGUG-CAGUG- -5' |
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| 17989 | 3' | -56.2 | NC_004665.1 | + | 18190 | 0.68 | 0.392416 |
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Target: 5'- aGGUCGuCGGCCuuGUGAaCCCCAaCGggaUCACu -3' miRNA: 3'- -UCAGC-GUCGG--UACUaGGGGU-GC---AGUG- -5' |
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| 17989 | 3' | -56.2 | NC_004665.1 | + | 30137 | 1.08 | 0.000488 |
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Target: 5'- cAGUCGCAGCCAUGAUCCCCACGUCACc -3' miRNA: 3'- -UCAGCGUCGGUACUAGGGGUGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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