Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17992 | 5' | -48.5 | NC_004665.1 | + | 3674 | 0.66 | 0.933112 |
Target: 5'- uGuCCAGUU-GGGAuuCGUgGAGCUg -3' miRNA: 3'- uCuGGUCAAuCCCUuuGCAaUUCGGa -5' |
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17992 | 5' | -48.5 | NC_004665.1 | + | 16001 | 0.68 | 0.867112 |
Target: 5'- uGGCCA--UAGGGGAcCGUUAGGUUa -3' miRNA: 3'- uCUGGUcaAUCCCUUuGCAAUUCGGa -5' |
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17992 | 5' | -48.5 | NC_004665.1 | + | 4779 | 0.68 | 0.849098 |
Target: 5'- uGGACCAGagccGGGAAGCGcUUGcccucacgggacAGCCa -3' miRNA: 3'- -UCUGGUCaau-CCCUUUGC-AAU------------UCGGa -5' |
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17992 | 5' | -48.5 | NC_004665.1 | + | 37029 | 1.08 | 0.003214 |
Target: 5'- gAGACCAGUUAGGGAAACGUUAAGCCUu -3' miRNA: 3'- -UCUGGUCAAUCCCUUUGCAAUUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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