Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17993 | 3' | -50.1 | NC_004665.1 | + | 16261 | 0.66 | 0.899554 |
Target: 5'- gCCCGcAAUGuagCCCUcUGAGuaGCCCu -3' miRNA: 3'- -GGGUaUUACua-GGGGuACUCuaCGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 18024 | 0.66 | 0.899554 |
Target: 5'- --aGUGAUGGUCaguaCCCucaAGAUGCCCu -3' miRNA: 3'- gggUAUUACUAG----GGGuacUCUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 25136 | 0.66 | 0.883508 |
Target: 5'- aCCCAUuggcacccgacgaGAUGAUgCCCA-GAGcguucgacagGCCCg -3' miRNA: 3'- -GGGUA-------------UUACUAgGGGUaCUCua--------CGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 22221 | 0.66 | 0.876237 |
Target: 5'- gCCCA-AcgGGUCUgCCGaGaAGGUGCCCg -3' miRNA: 3'- -GGGUaUuaCUAGG-GGUaC-UCUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 9355 | 0.67 | 0.859264 |
Target: 5'- aCCCAUGAagcgaaUGGUCgCCCcUucGAUGCCa -3' miRNA: 3'- -GGGUAUU------ACUAG-GGGuAcuCUACGGg -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 11296 | 0.67 | 0.840294 |
Target: 5'- aCCAcGAuUGGucggccuUCCUCGUGAGGcuUGCCCu -3' miRNA: 3'- gGGUaUU-ACU-------AGGGGUACUCU--ACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 19724 | 0.67 | 0.831826 |
Target: 5'- cCCCAgc---AUCCCCAgcGGcUGCCCg -3' miRNA: 3'- -GGGUauuacUAGGGGUacUCuACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 33750 | 0.67 | 0.831826 |
Target: 5'- ----cAGUGGUCCCCAa-AGGUGUCCc -3' miRNA: 3'- ggguaUUACUAGGGGUacUCUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 9335 | 0.67 | 0.822193 |
Target: 5'- aCCuu-GUGAgaCUCCGUGAgGAUGCUCg -3' miRNA: 3'- gGGuauUACUa-GGGGUACU-CUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 22572 | 0.68 | 0.791993 |
Target: 5'- aCCA--GUGAUCUCCAcGAGGUaaCCCg -3' miRNA: 3'- gGGUauUACUAGGGGUaCUCUAc-GGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 18947 | 0.68 | 0.791993 |
Target: 5'- cCCCGgu-UGAaCCCCAgc-GGUGUCCa -3' miRNA: 3'- -GGGUauuACUaGGGGUacuCUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 27798 | 0.69 | 0.749165 |
Target: 5'- cUCCAa---GAUCagCCAUGAgGGUGCCCa -3' miRNA: 3'- -GGGUauuaCUAGg-GGUACU-CUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 22518 | 0.69 | 0.749165 |
Target: 5'- cCUCGUGGaGAUCaCUgGUGAGucUGCCCg -3' miRNA: 3'- -GGGUAUUaCUAG-GGgUACUCu-ACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 9037 | 0.69 | 0.726894 |
Target: 5'- aCCAgcGAUGAUCgcgccgaugCCCAguccaaUGAGAUGCUCa -3' miRNA: 3'- gGGUa-UUACUAG---------GGGU------ACUCUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 30723 | 0.7 | 0.692723 |
Target: 5'- uCUCGgAGUG-UCCCCAUGAGcagcucggaucAUGUCCa -3' miRNA: 3'- -GGGUaUUACuAGGGGUACUC-----------UACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 14545 | 0.71 | 0.622949 |
Target: 5'- aCCCGUGAggaagcccguuUGAUCCCCGcUGAGAagaucGCUg -3' miRNA: 3'- -GGGUAUU-----------ACUAGGGGU-ACUCUa----CGGg -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 3957 | 0.71 | 0.622949 |
Target: 5'- aCCGUGAUGGUCUggUUGUGuGGAUGCUCg -3' miRNA: 3'- gGGUAUUACUAGG--GGUAC-UCUACGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 37326 | 0.72 | 0.542072 |
Target: 5'- cCCCAUca--GUCCCCAuUGAGAUuCCCa -3' miRNA: 3'- -GGGUAuuacUAGGGGU-ACUCUAcGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 220 | 0.72 | 0.542072 |
Target: 5'- cCCCAUca--GUCCCCAuUGAGAUuCCCa -3' miRNA: 3'- -GGGUAuuacUAGGGGU-ACUCUAcGGG- -5' |
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17993 | 3' | -50.1 | NC_004665.1 | + | 28964 | 0.73 | 0.501775 |
Target: 5'- aCCCGUAugccgaucaacuccgAUGAUUCCCuugaggacuucaAUGGGGUGCUCu -3' miRNA: 3'- -GGGUAU---------------UACUAGGGG------------UACUCUACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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