Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17993 | 5' | -54 | NC_004665.1 | + | 32356 | 0.66 | 0.703869 |
Target: 5'- aGAGGCGGUCUUAgcggcUUCAGAcgcagcucguugGGUgGCa -3' miRNA: 3'- gCUCCGCUAGAGU-----GAGUCU------------CCAgUGg -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 37148 | 0.68 | 0.591158 |
Target: 5'- aGAGGUGAccauucagauuUUUCugUCAGAuucuuaaaGGUCAUCc -3' miRNA: 3'- gCUCCGCU-----------AGAGugAGUCU--------CCAGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 32103 | 0.68 | 0.557584 |
Target: 5'- gCGAGGUugacGAUCUUGCgggcaCGGgcaucaAGGUCACCa -3' miRNA: 3'- -GCUCCG----CUAGAGUGa----GUC------UCCAGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 29859 | 0.69 | 0.535501 |
Target: 5'- -uGGGCGAUCa-GCUCAGGcGUUGCCa -3' miRNA: 3'- gcUCCGCUAGagUGAGUCUcCAGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 25495 | 0.69 | 0.492336 |
Target: 5'- cCGuuGGCGAUugcCUCacggcgcuGCUCAGGGGUCAgCu -3' miRNA: 3'- -GCu-CCGCUA---GAG--------UGAGUCUCCAGUgG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 31719 | 0.7 | 0.450844 |
Target: 5'- uCGAGu--AUCUCGCUCGGAaguucguuguGGUCACCu -3' miRNA: 3'- -GCUCcgcUAGAGUGAGUCU----------CCAGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 30047 | 0.71 | 0.392416 |
Target: 5'- cCGAGaacgaCGAgccaUCGCUCAGAGG-CACCg -3' miRNA: 3'- -GCUCc----GCUag--AGUGAGUCUCCaGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 22929 | 0.74 | 0.262459 |
Target: 5'- -uGGGUcGUCaUCACUCAG-GGUCGCCa -3' miRNA: 3'- gcUCCGcUAG-AGUGAGUCuCCAGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 37241 | 1.13 | 0.000459 |
Target: 5'- cCGAGGCGAUCUCACUCAGAGGUCACCg -3' miRNA: 3'- -GCUCCGCUAGAGUGAGUCUCCAGUGG- -5' |
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17993 | 5' | -54 | NC_004665.1 | + | 135 | 1.13 | 0.000459 |
Target: 5'- cCGAGGCGAUCUCACUCAGAGGUCACCg -3' miRNA: 3'- -GCUCCGCUAGAGUGAGUCUCCAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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