Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17994 | 5' | -52.9 | NC_004665.1 | + | 15999 | 0.66 | 0.727396 |
Target: 5'- uAUGGCCAUAGGGGaccguuagguuaucUCuGugGCCuuuucguuuaauggAGGGCc -3' miRNA: 3'- -UACUGGUAUCCCU--------------AG-CugUGG--------------UCUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 26694 | 0.66 | 0.722922 |
Target: 5'- -cGACCAUcGGuucGGUCuAC-CCAGAGCa -3' miRNA: 3'- uaCUGGUAuCC---CUAGcUGuGGUCUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 30314 | 0.66 | 0.711668 |
Target: 5'- -aGAUCGccGGGAUgGugACCAauGGGCa -3' miRNA: 3'- uaCUGGUauCCCUAgCugUGGU--CUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 1301 | 0.66 | 0.700328 |
Target: 5'- -aGGCCAUcacGGauuGGAUCGAaCACCAGAc- -3' miRNA: 3'- uaCUGGUA---UC---CCUAGCU-GUGGUCUcg -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 14854 | 0.67 | 0.673988 |
Target: 5'- cGUGACCcucUGGGGAcucaaGAaggcugcccgugcgUACCAGAGCg -3' miRNA: 3'- -UACUGGu--AUCCCUag---CU--------------GUGGUCUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 4397 | 0.67 | 0.642781 |
Target: 5'- cGUGGCCuucucuGGGGUCGGCGUCAaGGCc -3' miRNA: 3'- -UACUGGuau---CCCUAGCUGUGGUcUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 35940 | 0.7 | 0.482143 |
Target: 5'- -cGGCCcgAGGGGUCGAuCACaaggauuuucuGAGCg -3' miRNA: 3'- uaCUGGuaUCCCUAGCU-GUGgu---------CUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 16045 | 0.73 | 0.321301 |
Target: 5'- uAUGGCCAUAGugaGGGUUuuuaGGCAgCAGAGCg -3' miRNA: 3'- -UACUGGUAUC---CCUAG----CUGUgGUCUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 30381 | 0.75 | 0.233213 |
Target: 5'- gAUGACCAcuUGGGGAaccaaGAUcaaACCAGAGCa -3' miRNA: 3'- -UACUGGU--AUCCCUag---CUG---UGGUCUCG- -5' |
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17994 | 5' | -52.9 | NC_004665.1 | + | 490 | 1.09 | 0.000913 |
Target: 5'- gAUGACCAUAGGGAUCGACACCAGAGCc -3' miRNA: 3'- -UACUGGUAUCCCUAGCUGUGGUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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