Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17995 | 5' | -43.6 | NC_004665.1 | + | 7563 | 0.66 | 0.998059 |
Target: 5'- -uGCCUuucUUCGAGAaCGA--GGAUGGCa -3' miRNA: 3'- auUGGGu--AAGCUCU-GCUaaUUUACUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 5844 | 0.66 | 0.997611 |
Target: 5'- aGACCg---CGAGAUcGUUAAAUGGCu -3' miRNA: 3'- aUUGGguaaGCUCUGcUAAUUUACUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 23277 | 0.66 | 0.997611 |
Target: 5'- aGACCCGUggauagCGuGGACGGUguucGUGAUg -3' miRNA: 3'- aUUGGGUAa-----GC-UCUGCUAauu-UACUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 5125 | 0.66 | 0.997081 |
Target: 5'- cGACCC--UCGGuuGACGA--GGAUGGCa -3' miRNA: 3'- aUUGGGuaAGCU--CUGCUaaUUUACUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 28937 | 0.67 | 0.993897 |
Target: 5'- gAACUCGUUCGgcucAGACGAcucagcGUGACg -3' miRNA: 3'- aUUGGGUAAGC----UCUGCUaauu--UACUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 17079 | 0.69 | 0.984449 |
Target: 5'- cGACCU--UCGAGACGcucuuGGUGACc -3' miRNA: 3'- aUUGGGuaAGCUCUGCuaau-UUACUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 12848 | 0.72 | 0.917237 |
Target: 5'- cAGCCCAUUCGAGACccggcguGUaGACg -3' miRNA: 3'- aUUGGGUAAGCUCUGcuaauu-UA-CUG- -5' |
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17995 | 5' | -43.6 | NC_004665.1 | + | 1035 | 1.09 | 0.011221 |
Target: 5'- cUAACCCAUUCGAGACGAUUAAAUGACc -3' miRNA: 3'- -AUUGGGUAAGCUCUGCUAAUUUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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