Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17996 | 5' | -51.5 | NC_004665.1 | + | 31315 | 0.66 | 0.838643 |
Target: 5'- -gGACCgagguaCGCAC-AGCAGCAgCCG-GGu -3' miRNA: 3'- agUUGGa-----GCGUGuUCGUUGU-GGCaCC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 17467 | 0.66 | 0.838643 |
Target: 5'- aCGACUcCGUgGCAGGCAguggGCGCuCGUGGu -3' miRNA: 3'- aGUUGGaGCG-UGUUCGU----UGUG-GCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 14660 | 0.66 | 0.81964 |
Target: 5'- -uGAUCUacCGCGCAGGCAu--CCGUGa -3' miRNA: 3'- agUUGGA--GCGUGUUCGUuguGGCACc -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 25350 | 0.66 | 0.799765 |
Target: 5'- gCAGCCUucgggaCGUACAccgAGCAGCGCCa--- -3' miRNA: 3'- aGUUGGA------GCGUGU---UCGUUGUGGcacc -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 13244 | 0.67 | 0.779114 |
Target: 5'- -aGACCuUCGCugGGGCAccauACACCccGGu -3' miRNA: 3'- agUUGG-AGCGugUUCGU----UGUGGcaCC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 25410 | 0.67 | 0.768529 |
Target: 5'- aUCAuCCgcaaGCugAccccugAGCAGCGCCGUGa -3' miRNA: 3'- -AGUuGGag--CGugU------UCGUUGUGGCACc -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 21998 | 0.67 | 0.757788 |
Target: 5'- cCAAcCCUCGCACcgaccuGCGccucauCACUGUGGc -3' miRNA: 3'- aGUU-GGAGCGUGuu----CGUu-----GUGGCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 21036 | 0.68 | 0.72923 |
Target: 5'- -aGACCagCGCACcAGCAGCuucaggacgcagaccGCCGUGa -3' miRNA: 3'- agUUGGa-GCGUGuUCGUUG---------------UGGCACc -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 18803 | 0.68 | 0.702217 |
Target: 5'- cUCGGCCUCGgugaGC-AGCAGaccaGCCGUGu -3' miRNA: 3'- -AGUUGGAGCg---UGuUCGUUg---UGGCACc -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 35580 | 0.68 | 0.702217 |
Target: 5'- gCAGCCa-GCAUAGG-AGCcaGCCGUGGg -3' miRNA: 3'- aGUUGGagCGUGUUCgUUG--UGGCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 18617 | 0.68 | 0.687392 |
Target: 5'- -gGACCUCG-AUAAGCugacccaguuccugAACGCUGUGGc -3' miRNA: 3'- agUUGGAGCgUGUUCG--------------UUGUGGCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 16144 | 0.69 | 0.667859 |
Target: 5'- cUCAacGCCUCGUACcugaucGCcacuGGCAUCGUGGa -3' miRNA: 3'- -AGU--UGGAGCGUGuu----CG----UUGUGGCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 29703 | 0.7 | 0.610013 |
Target: 5'- uUCAGgUacagCGCACGGGCAGCAUCGUc- -3' miRNA: 3'- -AGUUgGa---GCGUGUUCGUUGUGGCAcc -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 21881 | 0.7 | 0.610012 |
Target: 5'- aUCAACCgggaCGCccaaGAGCAAUACuuCGUGGg -3' miRNA: 3'- -AGUUGGa---GCGug--UUCGUUGUG--GCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 27153 | 0.7 | 0.598462 |
Target: 5'- -gGGCCUCGCugAgaacccucaaugGGcCGACACCG-GGa -3' miRNA: 3'- agUUGGAGCGugU------------UC-GUUGUGGCaCC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 31058 | 0.71 | 0.519034 |
Target: 5'- -gGGCCUCGCaACGGGCuuCuuCGUGGc -3' miRNA: 3'- agUUGGAGCG-UGUUCGuuGugGCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 33251 | 0.71 | 0.507997 |
Target: 5'- uUCAGCCagagCGCACcAGCAacccggccaGCGuuGUGGa -3' miRNA: 3'- -AGUUGGa---GCGUGuUCGU---------UGUggCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 18585 | 0.72 | 0.49706 |
Target: 5'- aCGACUggUGCugAAGCAcuGgGCCGUGGu -3' miRNA: 3'- aGUUGGa-GCGugUUCGU--UgUGGCACC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 2314 | 0.72 | 0.475515 |
Target: 5'- aCAACCUCG-ACuGGCGugGCCG-GGu -3' miRNA: 3'- aGUUGGAGCgUGuUCGUugUGGCaCC- -5' |
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17996 | 5' | -51.5 | NC_004665.1 | + | 32056 | 0.73 | 0.432877 |
Target: 5'- gUCAACCUCGCugAuggcgugaacgacGGUGAUGCUGUGa -3' miRNA: 3'- -AGUUGGAGCGugU-------------UCGUUGUGGCACc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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