Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17997 | 3' | -53.5 | NC_004665.1 | + | 11625 | 0.66 | 0.660216 |
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Target: 5'- uGGCCcgUGAGGccGCUcUGAAGGCu- -3' miRNA: 3'- -CUGGuuGCUCCuuCGGaACUUCCGca -5' |
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| 17997 | 3' | -53.5 | NC_004665.1 | + | 17309 | 0.66 | 0.648671 |
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Target: 5'- aGACCaAACGAGaAGGUCUcgcugagGAAGGCGc -3' miRNA: 3'- -CUGG-UUGCUCcUUCGGAa------CUUCCGCa -5' |
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| 17997 | 3' | -53.5 | NC_004665.1 | + | 11987 | 0.68 | 0.568023 |
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Target: 5'- cGACCAucaAgGAGGAAaCCUcGAAGGCa- -3' miRNA: 3'- -CUGGU---UgCUCCUUcGGAaCUUCCGca -5' |
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| 17997 | 3' | -53.5 | NC_004665.1 | + | 6479 | 0.68 | 0.556653 |
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Target: 5'- aGCCAACGAGuaucuGGcCCUUGAGGGUc- -3' miRNA: 3'- cUGGUUGCUCcu---UC-GGAACUUCCGca -5' |
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| 17997 | 3' | -53.5 | NC_004665.1 | + | 10924 | 0.68 | 0.522975 |
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Target: 5'- aGACCAcUGAGGAAGUCUUcGAccGCGc -3' miRNA: 3'- -CUGGUuGCUCCUUCGGAA-CUucCGCa -5' |
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| 17997 | 3' | -53.5 | NC_004665.1 | + | 18437 | 0.69 | 0.479368 |
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Target: 5'- cGCCAGUGAacuGGAGGacacCCUUGggGGCGUc -3' miRNA: 3'- cUGGUUGCU---CCUUC----GGAACuuCCGCA- -5' |
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| 17997 | 3' | -53.5 | NC_004665.1 | + | 2164 | 1.08 | 0.000943 |
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Target: 5'- aGACCAACGAGGAAGCCUUGAAGGCGUg -3' miRNA: 3'- -CUGGUUGCUCCUUCGGAACUUCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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