Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17998 | 3' | -50.6 | NC_004665.1 | + | 12339 | 0.66 | 0.849557 |
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Target: 5'- aCGCAGGCgcucAGUGGGagccagacagucUCAGGGugGaAUGGa -3' miRNA: 3'- -GCGUUCG----UCACCU------------AGUUCCugU-UGCU- -5' |
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| 17998 | 3' | -50.6 | NC_004665.1 | + | 12252 | 0.67 | 0.790736 |
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Target: 5'- gGCGAGCGGUGGuagcgcaccUCAAGG-CAGgaGAa -3' miRNA: 3'- gCGUUCGUCACCu--------AGUUCCuGUUg-CU- -5' |
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| 17998 | 3' | -50.6 | NC_004665.1 | + | 2406 | 0.67 | 0.769491 |
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Target: 5'- gGUGAGcCGGUGGG-CAAGGACGG-GAu -3' miRNA: 3'- gCGUUC-GUCACCUaGUUCCUGUUgCU- -5' |
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| 17998 | 3' | -50.6 | NC_004665.1 | + | 5248 | 0.68 | 0.747602 |
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Target: 5'- aCGCAcaguGGCguacaGGUGGGUCAGGcuCAGCGGu -3' miRNA: 3'- -GCGU----UCG-----UCACCUAGUUCcuGUUGCU- -5' |
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| 17998 | 3' | -50.6 | NC_004665.1 | + | 17477 | 0.7 | 0.62063 |
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Target: 5'- gGCAGGCAGUGGGcgcUCGuGGucuCAACa- -3' miRNA: 3'- gCGUUCGUCACCU---AGUuCCu--GUUGcu -5' |
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| 17998 | 3' | -50.6 | NC_004665.1 | + | 2492 | 1.1 | 0.00149 |
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Target: 5'- gCGCAAGCAGUGGAUCAAGGACAACGAg -3' miRNA: 3'- -GCGUUCGUCACCUAGUUCCUGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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