Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17998 | 5' | -58.3 | NC_004665.1 | + | 18060 | 0.66 | 0.436455 |
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Target: 5'- -aUCGCUGUGCgCugGACCaaGCg--- -3' miRNA: 3'- gaAGCGACACGgGugCUGGg-CGagua -5' |
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| 17998 | 5' | -58.3 | NC_004665.1 | + | 35505 | 0.66 | 0.407485 |
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Target: 5'- -aUCGUUGUGacguaUCCACGGCCUuccaGCUCGc -3' miRNA: 3'- gaAGCGACAC-----GGGUGCUGGG----CGAGUa -5' |
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| 17998 | 5' | -58.3 | NC_004665.1 | + | 5996 | 0.67 | 0.37975 |
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Target: 5'- -cUCGCUGUGCCCuCGuCUgGCagaUCGUg -3' miRNA: 3'- gaAGCGACACGGGuGCuGGgCG---AGUA- -5' |
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| 17998 | 5' | -58.3 | NC_004665.1 | + | 26980 | 0.67 | 0.35936 |
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Target: 5'- -cUCGUUGUaaccgucguagagaGUCCgaGCGAUCCGCUCAa -3' miRNA: 3'- gaAGCGACA--------------CGGG--UGCUGGGCGAGUa -5' |
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| 17998 | 5' | -58.3 | NC_004665.1 | + | 8999 | 0.74 | 0.117398 |
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Target: 5'- -gUgGCUGUGCCCGUGACCUGUUCu- -3' miRNA: 3'- gaAgCGACACGGGUGCUGGGCGAGua -5' |
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| 17998 | 5' | -58.3 | NC_004665.1 | + | 2528 | 1.05 | 0.000549 |
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Target: 5'- uCUUCGCUGUGCCCACGACCCGCUCAUc -3' miRNA: 3'- -GAAGCGACACGGGUGCUGGGCGAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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