Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17999 | 3' | -50.6 | NC_004665.1 | + | 2143 | 0.66 | 0.883642 |
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Target: 5'- cGAUcaagCCG-GGUGacaUCGAgACCAACGAg -3' miRNA: 3'- -CUAa---GGUgCCAUg--AGCUgUGGUUGCU- -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 20378 | 0.66 | 0.875589 |
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Target: 5'- ----gCGCuGUACUCGGCACgAACGu -3' miRNA: 3'- cuaagGUGcCAUGAGCUGUGgUUGCu -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 31194 | 0.66 | 0.867256 |
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Target: 5'- --aUCCuCGGUGCUCcaaucggcGugGCCAACu- -3' miRNA: 3'- cuaAGGuGCCAUGAG--------CugUGGUUGcu -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 9639 | 0.66 | 0.840655 |
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Target: 5'- aGGUUCUccagACGGUugUCGuggauguCACCAuuGCGGu -3' miRNA: 3'- -CUAAGG----UGCCAugAGCu------GUGGU--UGCU- -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 9797 | 0.67 | 0.811856 |
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Target: 5'- --cUCCACGGUgaguucGCaaGACACCGAUa- -3' miRNA: 3'- cuaAGGUGCCA------UGagCUGUGGUUGcu -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 2445 | 0.68 | 0.77695 |
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Target: 5'- --aUCCACGGUGCgaacugugccggggUCGAUaagguugacuucaGCCAGCGc -3' miRNA: 3'- cuaAGGUGCCAUG--------------AGCUG-------------UGGUUGCu -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 29237 | 0.7 | 0.658113 |
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Target: 5'- cGUUCCACGGUGCagaccgagagUGGCuCCAAUGGa -3' miRNA: 3'- cUAAGGUGCCAUGa---------GCUGuGGUUGCU- -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 12142 | 0.71 | 0.576955 |
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Target: 5'- --cUCCACGGcUACUCGAugacccacuuCACCAAuCGGc -3' miRNA: 3'- cuaAGGUGCC-AUGAGCU----------GUGGUU-GCU- -5' |
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| 17999 | 3' | -50.6 | NC_004665.1 | + | 2834 | 1.1 | 0.001869 |
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Target: 5'- gGAUUCCACGGUACUCGACACCAACGAg -3' miRNA: 3'- -CUAAGGUGCCAUGAGCUGUGGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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