Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18000 | 3' | -58.2 | NC_004665.1 | + | 7414 | 0.66 | 0.3686 |
Target: 5'- cGAGGGUUGCCGaagcccuucucagGGUUGCcgaagucaGGCUUCUg -3' miRNA: 3'- uCUUCCGACGGU-------------UCGACGa-------CCGGAGA- -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 32599 | 0.67 | 0.343164 |
Target: 5'- uGAAGGUguucgcguUGCCcAGCuUGUcGGCCUCa -3' miRNA: 3'- uCUUCCG--------ACGGuUCG-ACGaCCGGAGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 24868 | 0.67 | 0.342309 |
Target: 5'- -cAGGGcCUGCauguugaccucagCGAGCUGCUuacgGGCCUCg -3' miRNA: 3'- ucUUCC-GACG-------------GUUCGACGA----CCGGAGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 12469 | 0.67 | 0.310197 |
Target: 5'- -uGAGGCUGCgAAGCcgGaUGGUCUCa -3' miRNA: 3'- ucUUCCGACGgUUCGa-CgACCGGAGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 11643 | 0.68 | 0.302341 |
Target: 5'- uGAAGGCUGCCAuugcGGCUacccaaGCUG-CCUa- -3' miRNA: 3'- uCUUCCGACGGU----UCGA------CGACcGGAga -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 35268 | 0.68 | 0.279697 |
Target: 5'- cAGAGGGCgagugGUCAGGCUugGCUGGaCC-Ca -3' miRNA: 3'- -UCUUCCGa----CGGUUCGA--CGACC-GGaGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 36057 | 0.68 | 0.277508 |
Target: 5'- uGAGGGCaacuucgGUgacgguauguacauCAAGCUGCUGGCCcCUg -3' miRNA: 3'- uCUUCCGa------CG--------------GUUCGACGACCGGaGA- -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 19317 | 0.68 | 0.272454 |
Target: 5'- cGAAGGCUucaGCCAgaugcgcgagcaAGCcaucaagGCUGGUCUCa -3' miRNA: 3'- uCUUCCGA---CGGU------------UCGa------CGACCGGAGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 9379 | 0.73 | 0.125098 |
Target: 5'- aAGAuGGaaGCCAAGUgGCUGGCCUUg -3' miRNA: 3'- -UCUuCCgaCGGUUCGaCGACCGGAGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 25395 | 0.74 | 0.107847 |
Target: 5'- cGAAGGCUGCacgcccgacuuaaCGAacuGCUGCaUGGCCUCa -3' miRNA: 3'- uCUUCCGACG-------------GUU---CGACG-ACCGGAGa -5' |
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18000 | 3' | -58.2 | NC_004665.1 | + | 3158 | 1.08 | 0.000256 |
Target: 5'- cAGAAGGCUGCCAAGCUGCUGGCCUCUg -3' miRNA: 3'- -UCUUCCGACGGUUCGACGACCGGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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