Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18000 | 5' | -53.9 | NC_004665.1 | + | 26251 | 0.66 | 0.658912 |
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Target: 5'- uGGAG-CCAGccguucuucgcccGCAGGUCUUGGAGCa-- -3' miRNA: 3'- -CUUCuGGUU-------------CGUCCAGGACUUCGgac -5' |
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| 18000 | 5' | -53.9 | NC_004665.1 | + | 17922 | 0.66 | 0.648475 |
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Target: 5'- cAAGcCCGAGCAGGagCUgGAGGUCUa -3' miRNA: 3'- cUUCuGGUUCGUCCagGA-CUUCGGAc -5' |
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| 18000 | 5' | -53.9 | NC_004665.1 | + | 16919 | 0.66 | 0.629893 |
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Target: 5'- uGAGGCCAcguuggagcuucauGCAGGUUcccgCUGggGCCUc -3' miRNA: 3'- cUUCUGGUu-------------CGUCCAG----GACuuCGGAc -5' |
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| 18000 | 5' | -53.9 | NC_004665.1 | + | 4444 | 0.69 | 0.426459 |
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Target: 5'- aGAAGGUCGAGCAGGcgCUUGAAGCa-- -3' miRNA: 3'- -CUUCUGGUUCGUCCa-GGACUUCGgac -5' |
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| 18000 | 5' | -53.9 | NC_004665.1 | + | 16594 | 0.71 | 0.341896 |
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Target: 5'- cGggGACCGAGC-GGUCUuucgUGAGcGUCUGa -3' miRNA: 3'- -CuuCUGGUUCGuCCAGG----ACUU-CGGAC- -5' |
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| 18000 | 5' | -53.9 | NC_004665.1 | + | 3198 | 1.08 | 0.000831 |
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Target: 5'- aGAAGACCAAGCAGGUCCUGAAGCCUGc -3' miRNA: 3'- -CUUCUGGUUCGUCCAGGACUUCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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