miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18000 5' -53.9 NC_004665.1 + 26251 0.66 0.658912
Target:  5'- uGGAG-CCAGccguucuucgcccGCAGGUCUUGGAGCa-- -3'
miRNA:   3'- -CUUCuGGUU-------------CGUCCAGGACUUCGgac -5'
18000 5' -53.9 NC_004665.1 + 17922 0.66 0.648475
Target:  5'- cAAGcCCGAGCAGGagCUgGAGGUCUa -3'
miRNA:   3'- cUUCuGGUUCGUCCagGA-CUUCGGAc -5'
18000 5' -53.9 NC_004665.1 + 16919 0.66 0.629893
Target:  5'- uGAGGCCAcguuggagcuucauGCAGGUUcccgCUGggGCCUc -3'
miRNA:   3'- cUUCUGGUu-------------CGUCCAG----GACuuCGGAc -5'
18000 5' -53.9 NC_004665.1 + 4444 0.69 0.426459
Target:  5'- aGAAGGUCGAGCAGGcgCUUGAAGCa-- -3'
miRNA:   3'- -CUUCUGGUUCGUCCa-GGACUUCGgac -5'
18000 5' -53.9 NC_004665.1 + 16594 0.71 0.341896
Target:  5'- cGggGACCGAGC-GGUCUuucgUGAGcGUCUGa -3'
miRNA:   3'- -CuuCUGGUUCGuCCAGG----ACUU-CGGAC- -5'
18000 5' -53.9 NC_004665.1 + 3198 1.08 0.000831
Target:  5'- aGAAGACCAAGCAGGUCCUGAAGCCUGc -3'
miRNA:   3'- -CUUCUGGUUCGUCCAGGACUUCGGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.