miRNA: 3'- uGGGCACUCUcGCGGcaUACCUAGUG- -5'
miRNA: 3'- ugGGCACUC-UCgCGGCaUACCUAGUg -5'
miRNA: 3'- -UGGgcACUCuCGC-GGCAUACCUaGUG- -5'
miRNA: 3'- -UGGGcACUC-UCGCGGCAUaCC--UAGUG- -5'
miRNA: 3'- -UGGGCACUCUCGCGGCAUACCUAGUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18002 | 5' | -55.9 | NC_004665.1 | + | 9346 | 0.66 | 0.526505 |
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Target: 5'- cUCCGUGAGGaUGCUc-GUGGGUCGCg -3' miRNA: 3'- uGGGCACUCUcGCGGcaUACCUAGUG- -5' |
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| 18002 | 5' | -55.9 | NC_004665.1 | + | 22148 | 0.68 | 0.442605 |
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Target: 5'- -gCCGUGGGuAG-GCCGcAUGGGUCAg -3' miRNA: 3'- ugGGCACUC-UCgCGGCaUACCUAGUg -5' |
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| 18002 | 5' | -55.9 | NC_004665.1 | + | 12916 | 0.69 | 0.357956 |
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Target: 5'- aGCCauUGAGuGUGuCCGUAUGGAgCACg -3' miRNA: 3'- -UGGgcACUCuCGC-GGCAUACCUaGUG- -5' |
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| 18002 | 5' | -55.9 | NC_004665.1 | + | 25424 | 0.73 | 0.212947 |
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Target: 5'- gACCCcUGAGcAGCGCCGUGaGGcaAUCGCc -3' miRNA: 3'- -UGGGcACUC-UCGCGGCAUaCC--UAGUG- -5' |
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| 18002 | 5' | -55.9 | NC_004665.1 | + | 3931 | 1.08 | 0.000575 |
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Target: 5'- aACCCGUGAGAGCGCCGUAUGGAUCACc -3' miRNA: 3'- -UGGGCACUCUCGCGGCAUACCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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