Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18003 | 5' | -57 | NC_004665.1 | + | 17927 | 0.66 | 0.472244 |
Target: 5'- -cCGAGCAGGaGCUgGAGGUcuauACCCa- -3' miRNA: 3'- caGCUCGUCCgCGAaCUUCG----UGGGcg -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 29706 | 0.66 | 0.472244 |
Target: 5'- aGUCGAGCuggcuGGCGUccuacGCuuCCCGCg -3' miRNA: 3'- -CAGCUCGu----CCGCGaacuuCGu-GGGCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 18575 | 0.67 | 0.431841 |
Target: 5'- gGUCGcuuGCAGuuGgUUGAgcAGCACUCGCa -3' miRNA: 3'- -CAGCu--CGUCcgCgAACU--UCGUGGGCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 27946 | 0.68 | 0.393529 |
Target: 5'- aGUUGAGCuuuGGgGCUUGggGauuCCCa- -3' miRNA: 3'- -CAGCUCGu--CCgCGAACuuCgu-GGGcg -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 18569 | 0.68 | 0.369839 |
Target: 5'- uGUCGAGCcgaccguaacgacuGGUGC-UGAAGCACugggCCGUg -3' miRNA: 3'- -CAGCUCGu-------------CCGCGaACUUCGUG----GGCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 22444 | 0.69 | 0.339521 |
Target: 5'- uGUCGuuGGCAGGgGCaUUGAugagaauccacccGGUGCCUGCu -3' miRNA: 3'- -CAGC--UCGUCCgCG-AACU-------------UCGUGGGCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 12808 | 0.73 | 0.185791 |
Target: 5'- uUCGAGCuGGCGCaUGAAGUcguGCuuGUa -3' miRNA: 3'- cAGCUCGuCCGCGaACUUCG---UGggCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 26236 | 0.74 | 0.144417 |
Target: 5'- uUCGcccGCAGGU-CUUGGAGCAUCCGCc -3' miRNA: 3'- cAGCu--CGUCCGcGAACUUCGUGGGCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 4520 | 0.75 | 0.118249 |
Target: 5'- gGUCuucuuGGCGGGUGCUUcAAGCGCCUGCu -3' miRNA: 3'- -CAGc----UCGUCCGCGAAcUUCGUGGGCG- -5' |
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18003 | 5' | -57 | NC_004665.1 | + | 4448 | 1.1 | 0.0003 |
Target: 5'- gGUCGAGCAGGCGCUUGAAGCACCCGCc -3' miRNA: 3'- -CAGCUCGUCCGCGAACUUCGUGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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