Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18004 | 5' | -51.9 | NC_004665.1 | + | 13675 | 0.66 | 0.802986 |
Target: 5'- gUCGUCCaagUGGGCACCGaugcgagcGGCCuugagCUGCg -3' miRNA: 3'- -AGUAGG---ACUUGUGGU--------UCGGcaa--GGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 1329 | 0.66 | 0.782482 |
Target: 5'- aUCGggaCCaGAugACCGAGCagcgGUUUCGCu -3' miRNA: 3'- -AGUa--GGaCUugUGGUUCGg---CAAGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 30710 | 0.66 | 0.782482 |
Target: 5'- aUCG-CCUGucgauCACCAAGCaacaGUUCgGUg -3' miRNA: 3'- -AGUaGGACuu---GUGGUUCGg---CAAGgCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 5367 | 0.66 | 0.771965 |
Target: 5'- aCAUCCUGAAUgaaGCCAuccucguuGUCG-UCCGg -3' miRNA: 3'- aGUAGGACUUG---UGGUu-------CGGCaAGGCg -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 20457 | 0.66 | 0.771965 |
Target: 5'- gUUGUUCUGAACAUCGGuGCCG--CUGCu -3' miRNA: 3'- -AGUAGGACUUGUGGUU-CGGCaaGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 5921 | 0.67 | 0.765577 |
Target: 5'- cUCGUCaccaaGGGCACCuuugcgcaaucccgaAAGCUGggCCGCa -3' miRNA: 3'- -AGUAGga---CUUGUGG---------------UUCGGCaaGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 7286 | 0.67 | 0.761289 |
Target: 5'- --uUCCUGuucacuccagucGGCACCGcugAGCCGUaCUGCu -3' miRNA: 3'- aguAGGAC------------UUGUGGU---UCGGCAaGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 7707 | 0.67 | 0.739511 |
Target: 5'- cCAUCgCUGGcgguuCCAAGCUGaaggUCCGCu -3' miRNA: 3'- aGUAG-GACUugu--GGUUCGGCa---AGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 26499 | 0.67 | 0.728436 |
Target: 5'- aUCuUCCUGAcaacgugaaggACGCCAAGC-GUgCUGCa -3' miRNA: 3'- -AGuAGGACU-----------UGUGGUUCGgCAaGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 25517 | 0.67 | 0.717255 |
Target: 5'- gUCAUCCUGAuACAgCAAgguGCCG-UUgGCg -3' miRNA: 3'- -AGUAGGACU-UGUgGUU---CGGCaAGgCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 34457 | 0.68 | 0.68206 |
Target: 5'- aCAcCCUGAccaucaaACACCAAGCgGaaCUGCu -3' miRNA: 3'- aGUaGGACU-------UGUGGUUCGgCaaGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 32391 | 0.68 | 0.671731 |
Target: 5'- uUCGUCCUac-CACUAcAGCCGUaagUCUGCu -3' miRNA: 3'- -AGUAGGAcuuGUGGU-UCGGCA---AGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 26674 | 0.68 | 0.660216 |
Target: 5'- cUCA-CCaGAgcGCACCGAGuuGUUCCa- -3' miRNA: 3'- -AGUaGGaCU--UGUGGUUCggCAAGGcg -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 13082 | 0.69 | 0.63711 |
Target: 5'- aUCAgCCgaaagGuGGCACCGAGCaGUUCCGUc -3' miRNA: 3'- -AGUaGGa----C-UUGUGGUUCGgCAAGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 3359 | 0.69 | 0.63711 |
Target: 5'- gUCG-CCgagGAACAUCAGGUCGaUUCGCc -3' miRNA: 3'- -AGUaGGa--CUUGUGGUUCGGCaAGGCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 845 | 0.69 | 0.625544 |
Target: 5'- -gGUCUUGugugaGCCAAGCCGUaCaCGCa -3' miRNA: 3'- agUAGGACuug--UGGUUCGGCAaG-GCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 22926 | 0.7 | 0.545349 |
Target: 5'- -gAUCCUGAaauACGCUguGCCGUUCUcugGCa -3' miRNA: 3'- agUAGGACU---UGUGGuuCGGCAAGG---CG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 4820 | 0.72 | 0.468742 |
Target: 5'- uUCAUgCUGGACGCUGAGuuGaUCCuGCu -3' miRNA: 3'- -AGUAgGACUUGUGGUUCggCaAGG-CG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 10588 | 0.72 | 0.437618 |
Target: 5'- cCAUCUgGAAUGCCcAGCCGUUCgUGCc -3' miRNA: 3'- aGUAGGaCUUGUGGuUCGGCAAG-GCG- -5' |
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18004 | 5' | -51.9 | NC_004665.1 | + | 15454 | 0.73 | 0.41754 |
Target: 5'- gCAUCCUGAugACCAAucCCGgUCuCGCu -3' miRNA: 3'- aGUAGGACUugUGGUUc-GGCaAG-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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