miRNA: 3'- aaGGGAGCACCaGCaGUGG--CUGuACG- -5'
miRNA: 3'- aaGGGAGC-ACCAGCaGugGCUGuACG- -5'
miRNA: 3'- aAGGGAGCACCa-----GC-AGUgGCUGUACG- -5'
miRNA: 3'- -AAGGGAGCACCAGCAGUGGCUGUACG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18005 | 5' | -55.4 | NC_004665.1 | + | 24641 | 0.66 | 0.561476 |
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Target: 5'- -gCCCUCGUGGgCGgCACUauGGC-UGCg -3' miRNA: 3'- aaGGGAGCACCaGCaGUGG--CUGuACG- -5' |
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| 18005 | 5' | -55.4 | NC_004665.1 | + | 12037 | 0.68 | 0.496142 |
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Target: 5'- -cUCCUUGaUGGUCG-CugCGACcUGCu -3' miRNA: 3'- aaGGGAGC-ACCAGCaGugGCUGuACG- -5' |
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| 18005 | 5' | -55.4 | NC_004665.1 | + | 22746 | 0.68 | 0.475116 |
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Target: 5'- gUCCUUCGUGGgcgcuaCGaUCAaCGACGUGUu -3' miRNA: 3'- aAGGGAGCACCa-----GC-AGUgGCUGUACG- -5' |
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| 18005 | 5' | -55.4 | NC_004665.1 | + | 6448 | 1.1 | 0.000483 |
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Target: 5'- aUUCCCUCGUGGUCGUCACCGACAUGCg -3' miRNA: 3'- -AAGGGAGCACCAGCAGUGGCUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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