Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18008 | 3' | -55 | NC_004665.1 | + | 27666 | 0.67 | 0.56342 |
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Target: 5'- -gGUCAucaGCCCGUAAGCCUugccGGUCGc -3' miRNA: 3'- cgCAGUca-UGGGCGUUCGGGu---CUAGC- -5' |
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| 18008 | 3' | -55 | NC_004665.1 | + | 17706 | 0.67 | 0.552333 |
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Target: 5'- cCGUCAGUugGCCCuuGCAGGCaCCGcucuGAUCu -3' miRNA: 3'- cGCAGUCA--UGGG--CGUUCG-GGU----CUAGc -5' |
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| 18008 | 3' | -55 | NC_004665.1 | + | 15881 | 0.68 | 0.530366 |
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Target: 5'- uGCGagGGacGCCaUGCAGGCCCAGAggUCa -3' miRNA: 3'- -CGCagUCa-UGG-GCGUUCGGGUCU--AGc -5' |
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| 18008 | 3' | -55 | NC_004665.1 | + | 15359 | 0.69 | 0.466637 |
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Target: 5'- -aGUCGGcUACCCGCAcuucuGCCaagGGAUCa -3' miRNA: 3'- cgCAGUC-AUGGGCGUu----CGGg--UCUAGc -5' |
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| 18008 | 3' | -55 | NC_004665.1 | + | 18030 | 0.7 | 0.407099 |
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Target: 5'- -gGUCAGUACCCuCAAgauGCCCugccguGGAUCGc -3' miRNA: 3'- cgCAGUCAUGGGcGUU---CGGG------UCUAGC- -5' |
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| 18008 | 3' | -55 | NC_004665.1 | + | 8745 | 1.11 | 0.00052 |
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Target: 5'- aGCGUCAGUACCCGCAAGCCCAGAUCGu -3' miRNA: 3'- -CGCAGUCAUGGGCGUUCGGGUCUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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