Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18008 | 5' | -54.8 | NC_004665.1 | + | 19858 | 0.66 | 0.578947 |
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Target: 5'- gGCG-CUUCGAg-CUGGUAAGGCaCUGa -3' miRNA: 3'- gUGCuGGAGCUgaGACCGUUUCG-GAC- -5' |
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| 18008 | 5' | -54.8 | NC_004665.1 | + | 4585 | 0.66 | 0.578947 |
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Target: 5'- gACGGCUUCGACUUUGGaCAuuGGGUUg- -3' miRNA: 3'- gUGCUGGAGCUGAGACC-GU--UUCGGac -5' |
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| 18008 | 5' | -54.8 | NC_004665.1 | + | 4056 | 0.66 | 0.578947 |
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Target: 5'- aCACGaACCUC-ACg--GGcCAGAGCCUGu -3' miRNA: 3'- -GUGC-UGGAGcUGagaCC-GUUUCGGAC- -5' |
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| 18008 | 5' | -54.8 | NC_004665.1 | + | 20523 | 0.69 | 0.409192 |
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Target: 5'- aAgGGCCU-GACUCUGGCucgugcucGCCUGa -3' miRNA: 3'- gUgCUGGAgCUGAGACCGuuu-----CGGAC- -5' |
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| 18008 | 5' | -54.8 | NC_004665.1 | + | 25557 | 0.7 | 0.362735 |
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Target: 5'- aGCGACC-CGACuUCUGGCGcAGCa-- -3' miRNA: 3'- gUGCUGGaGCUG-AGACCGUuUCGgac -5' |
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| 18008 | 5' | -54.8 | NC_004665.1 | + | 8779 | 1.07 | 0.000876 |
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Target: 5'- uCACGACCUCGACUCUGGCAAAGCCUGc -3' miRNA: 3'- -GUGCUGGAGCUGAGACCGUUUCGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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