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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18011 | 3' | -49.6 | NC_004665.1 | + | 10103 | 0.66 | 0.91019 |
Target: 5'- gGUGggUCgugUCUgGcACCCGAUCAagcgUGAg -3' miRNA: 3'- -CACuuAGg--AGAgC-UGGGCUAGUa---ACU- -5' |
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18011 | 3' | -49.6 | NC_004665.1 | + | 5087 | 0.66 | 0.880052 |
Target: 5'- aUGAggUCUaCUCGAUggaaaccaugCCGGUCAUUGAg -3' miRNA: 3'- cACUuaGGA-GAGCUG----------GGCUAGUAACU- -5' |
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18011 | 3' | -49.6 | NC_004665.1 | + | 11926 | 1.1 | 0.002189 |
Target: 5'- uGUGAAUCCUCUCGACCCGAUCAUUGAg -3' miRNA: 3'- -CACUUAGGAGAGCUGGGCUAGUAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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