Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18012 | 3' | -58 | NC_004665.1 | + | 31262 | 0.68 | 0.350817 |
Target: 5'- uGUGCgUACCU-CGGUCCUCccACgagGCCc -3' miRNA: 3'- uCACG-GUGGAuGCCAGGAG--UGa--CGGc -5' |
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18012 | 3' | -58 | NC_004665.1 | + | 1118 | 0.71 | 0.221537 |
Target: 5'- -aUGCCuugagcaacAUCUACGGUCCugcacUCGCUGCUGa -3' miRNA: 3'- ucACGG---------UGGAUGCCAGG-----AGUGACGGC- -5' |
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18012 | 3' | -58 | NC_004665.1 | + | 24843 | 0.71 | 0.221537 |
Target: 5'- --aGCUGCUUACGGgCCUCGuCUGCCu -3' miRNA: 3'- ucaCGGUGGAUGCCaGGAGU-GACGGc -5' |
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18012 | 3' | -58 | NC_004665.1 | + | 13599 | 1.08 | 0.00036 |
Target: 5'- gAGUGCCACCUACGGUCCUCACUGCCGu -3' miRNA: 3'- -UCACGGUGGAUGCCAGGAGUGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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