Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18013 | 5' | -50.5 | NC_004665.1 | + | 23860 | 0.66 | 0.868595 |
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Target: 5'- uGCAAGCGuccgauguccucggUCGAgGUGGAC-CCAu -3' miRNA: 3'- gCGUUCGCuga-----------AGCUaUACCUGuGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 18856 | 0.66 | 0.862585 |
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Target: 5'- cCGCAAGUcccgaggcaaugGAgUcCGcUAUGGACACCGc -3' miRNA: 3'- -GCGUUCG------------CUgAaGCuAUACCUGUGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 26107 | 0.66 | 0.853768 |
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Target: 5'- cCGCAcccGUGAgUUCGAgcUGGGCAUUAc -3' miRNA: 3'- -GCGUu--CGCUgAAGCUauACCUGUGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 13239 | 0.66 | 0.835355 |
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Target: 5'- gCGCGAGac-CUUCGcug-GGGCACCAu -3' miRNA: 3'- -GCGUUCgcuGAAGCuauaCCUGUGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 9359 | 0.67 | 0.784245 |
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Target: 5'- uCGCAcccaugaAGCGAauggucgcccCUUCGAUGccacguUGGAUGCCAg -3' miRNA: 3'- -GCGU-------UCGCU----------GAAGCUAU------ACCUGUGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 6589 | 0.71 | 0.59159 |
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Target: 5'- aGCugcGCGACcaCGuUAUGGACGCCGu -3' miRNA: 3'- gCGuu-CGCUGaaGCuAUACCUGUGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 17086 | 0.72 | 0.500499 |
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Target: 5'- aCGCcAGCGAcCUUCGAgacgcucuUGGugACCGa -3' miRNA: 3'- -GCGuUCGCU-GAAGCUau------ACCugUGGU- -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 14888 | 0.74 | 0.436422 |
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Target: 5'- gCGUaccaGAGCGACUUCGuucGUGGGCGCa- -3' miRNA: 3'- -GCG----UUCGCUGAAGCua-UACCUGUGgu -5' |
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| 18013 | 5' | -50.5 | NC_004665.1 | + | 15907 | 1.09 | 0.001801 |
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Target: 5'- gCGCAAGCGACUUCGAUAUGGACACCAu -3' miRNA: 3'- -GCGUUCGCUGAAGCUAUACCUGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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