Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18014 | 5' | -49.4 | NC_004665.1 | + | 30162 | 0.66 | 0.906349 |
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Target: 5'- -cUCUgaCGugGAGGCCcUUGAGUCu -3' miRNA: 3'- gaAGAgaGCuuUUCCGGuAGCUCAGc -5' |
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| 18014 | 5' | -49.4 | NC_004665.1 | + | 12697 | 0.67 | 0.88375 |
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Target: 5'- -cUCUCUgGAAGcgugGGGCUAUCGccUCGg -3' miRNA: 3'- gaAGAGAgCUUU----UCCGGUAGCucAGC- -5' |
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| 18014 | 5' | -49.4 | NC_004665.1 | + | 6104 | 0.7 | 0.747601 |
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Target: 5'- gCUUCUCcgUGAGgaaggcuucGAGGuCUAUCGGGUCGc -3' miRNA: 3'- -GAAGAGa-GCUU---------UUCC-GGUAGCUCAGC- -5' |
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| 18014 | 5' | -49.4 | NC_004665.1 | + | 33032 | 0.7 | 0.747601 |
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Target: 5'- -gUCUUUCGGAAAcGCCAUUGAGg-- -3' miRNA: 3'- gaAGAGAGCUUUUcCGGUAGCUCagc -5' |
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| 18014 | 5' | -49.4 | NC_004665.1 | + | 35732 | 0.72 | 0.608905 |
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Target: 5'- aCUUCUCcaugUCGGAGAGGU--UCGGGUUGa -3' miRNA: 3'- -GAAGAG----AGCUUUUCCGguAGCUCAGC- -5' |
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| 18014 | 5' | -49.4 | NC_004665.1 | + | 19600 | 1.09 | 0.00273 |
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Target: 5'- gCUUCUCUCGAAAAGGCCAUCGAGUCGc -3' miRNA: 3'- -GAAGAGAGCUUUUCCGGUAGCUCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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