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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18015 | 3' | -59.3 | NC_004665.1 | + | 26520 | 0.66 | 0.359387 |
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Target: 5'- aCGCCAAGCGUGCUgcauaCCuGCGUgCUg- -3' miRNA: 3'- aGUGGUUCGCACGG-----GGuCGUAgGGgu -5' |
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| 18015 | 3' | -59.3 | NC_004665.1 | + | 6426 | 0.67 | 0.334116 |
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Target: 5'- cUCAUCGAGaagGCCCCAGaggAUUCCCu -3' miRNA: 3'- -AGUGGUUCgcaCGGGGUCg--UAGGGGu -5' |
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| 18015 | 3' | -59.3 | NC_004665.1 | + | 18965 | 0.67 | 0.334116 |
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Target: 5'- -aACCugaguGGCGauugGCCCCGGUugaaCCCCAg -3' miRNA: 3'- agUGGu----UCGCa---CGGGGUCGua--GGGGU- -5' |
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| 18015 | 3' | -59.3 | NC_004665.1 | + | 14310 | 0.71 | 0.168412 |
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Target: 5'- gUCACCGGGCGUGCCaucguccuuguaCCAGCcgUgguaaagCCCAu -3' miRNA: 3'- -AGUGGUUCGCACGG------------GGUCGuaG-------GGGU- -5' |
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| 18015 | 3' | -59.3 | NC_004665.1 | + | 19711 | 1.09 | 0.000215 |
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Target: 5'- gUCACCAAGCGUGCCCCAGCAUCCCCAg -3' miRNA: 3'- -AGUGGUUCGCACGGGGUCGUAGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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