Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18015 | 5' | -54.2 | NC_004665.1 | + | 35568 | 0.66 | 0.705709 |
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Target: 5'- uGUucuGGGCACCGacugaCGAGGuucGCUUCGa -3' miRNA: 3'- gCAuu-CUCGUGGCa----GCUCC---CGAAGCg -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 25011 | 0.66 | 0.705709 |
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Target: 5'- --cAAGAGCGCCcUCaAGGGUcaagCGCa -3' miRNA: 3'- gcaUUCUCGUGGcAGcUCCCGaa--GCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 36845 | 0.67 | 0.626995 |
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Target: 5'- uGUAGGuGgACCGagaGAGGGCaaggCGCu -3' miRNA: 3'- gCAUUCuCgUGGCag-CUCCCGaa--GCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 21681 | 0.67 | 0.626995 |
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Target: 5'- aCGUAAGAGuCACCGUCGAucauGUUgaaacCGCc -3' miRNA: 3'- -GCAUUCUC-GUGGCAGCUcc--CGAa----GCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 16734 | 0.68 | 0.537439 |
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Target: 5'- uCGUGAGAGaUGCCucugggaagcUCGuGGGUUUCGCu -3' miRNA: 3'- -GCAUUCUC-GUGGc---------AGCuCCCGAAGCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 8566 | 0.69 | 0.526505 |
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Target: 5'- uCGUAAcGGCACCGUCGAGaaCggUCGUc -3' miRNA: 3'- -GCAUUcUCGUGGCAGCUCccGa-AGCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 22688 | 0.69 | 0.504899 |
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Target: 5'- uGUGGGAGCGuuGcguuguugaacccugCGAugaucuuGGGCUUCGCu -3' miRNA: 3'- gCAUUCUCGUggCa--------------GCU-------CCCGAAGCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 27124 | 0.77 | 0.156926 |
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Target: 5'- gGUcAGAGCAUCGUCGAggacaccuugaaGGGCcUCGCu -3' miRNA: 3'- gCAuUCUCGUGGCAGCU------------CCCGaAGCG- -5' |
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| 18015 | 5' | -54.2 | NC_004665.1 | + | 19746 | 1.12 | 0.000525 |
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Target: 5'- cCGUAAGAGCACCGUCGAGGGCUUCGCu -3' miRNA: 3'- -GCAUUCUCGUGGCAGCUCCCGAAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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