Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18016 | 5' | -53.4 | NC_004665.1 | + | 13694 | 0.66 | 0.711588 |
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Target: 5'- aUGCGAGCggccuugaGCUGCGUUGcUUGGg -3' miRNA: 3'- gACGCUUGaug-----CGACGCGACuAGCUg -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 33936 | 0.66 | 0.700297 |
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Target: 5'- gUGCGAGCUGCcccuaucgccGCUGgaGCUGGUgcCGAa -3' miRNA: 3'- gACGCUUGAUG----------CGACg-CGACUA--GCUg -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 4353 | 0.67 | 0.654531 |
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Target: 5'- -aGCGGACUgGCGCaGgGCU--UCGACa -3' miRNA: 3'- gaCGCUUGA-UGCGaCgCGAcuAGCUG- -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 17137 | 0.67 | 0.631462 |
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Target: 5'- -aGCGAACgcUGCUGCugUGGUCGAa -3' miRNA: 3'- gaCGCUUGauGCGACGcgACUAGCUg -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 20968 | 0.67 | 0.59689 |
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Target: 5'- uCUGCGuccugaAGCUGCuGgUGCGCUGGUCu-- -3' miRNA: 3'- -GACGC------UUGAUG-CgACGCGACUAGcug -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 35858 | 0.71 | 0.375078 |
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Target: 5'- aCUGCGAGCUACGCUcaGaaaauccuugUGAUCGACc -3' miRNA: 3'- -GACGCUUGAUGCGAcgCg---------ACUAGCUG- -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 19264 | 0.74 | 0.261412 |
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Target: 5'- -gGCGAGCcugAUGCUGaGCUGGUCGAa -3' miRNA: 3'- gaCGCUUGa--UGCGACgCGACUAGCUg -5' |
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| 18016 | 5' | -53.4 | NC_004665.1 | + | 20640 | 1.09 | 0.000885 |
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Target: 5'- gCUGCGAACUACGCUGCGCUGAUCGACc -3' miRNA: 3'- -GACGCUUGAUGCGACGCGACUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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