miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18017 3' -55.1 NC_004665.1 + 14138 0.66 0.623628
Target:  5'- -cUCUGCGuugaacuUCCUGcuccAGuCUGCUGGcGCa -3'
miRNA:   3'- ccAGACGC-------AGGACu---UC-GACGACCaCG- -5'
18017 3' -55.1 NC_004665.1 + 25300 0.66 0.602045
Target:  5'- uGGUCgcaGUGgacCCgUGGAGCUGCUcaGUGCc -3'
miRNA:   3'- -CCAGa--CGCa--GG-ACUUCGACGAc-CACG- -5'
18017 3' -55.1 NC_004665.1 + 25428 0.66 0.624766
Target:  5'- cGG-CUGUcUUUUGgcGCUGCUcGGUGUa -3'
miRNA:   3'- -CCaGACGcAGGACuuCGACGA-CCACG- -5'
18017 3' -55.1 NC_004665.1 + 17037 0.68 0.480935
Target:  5'- aGGUCgcugGCGUagccUCUGGuGGcCUGCUGGgugGCa -3'
miRNA:   3'- -CCAGa---CGCA----GGACU-UC-GACGACCa--CG- -5'
18017 3' -55.1 NC_004665.1 + 32689 0.69 0.439783
Target:  5'- aGGUCUucuaCGUUCgcu-GUUGCUGGUGCc -3'
miRNA:   3'- -CCAGAc---GCAGGacuuCGACGACCACG- -5'
18017 3' -55.1 NC_004665.1 + 11281 0.71 0.337065
Target:  5'- cGGUCUGCGUCaaCUGAGugacaccaucaucGC-GCUGGaGCg -3'
miRNA:   3'- -CCAGACGCAG--GACUU-------------CGaCGACCaCG- -5'
18017 3' -55.1 NC_004665.1 + 20965 1.15 0.000218
Target:  5'- cGGUCUGCGUCCUGAAGCUGCUGGUGCg -3'
miRNA:   3'- -CCAGACGCAGGACUUCGACGACCACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.