Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18018 | 3' | -55.9 | NC_004665.1 | + | 17258 | 0.66 | 0.566236 |
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Target: 5'- -aGCcuucuUCGCAGCu-UCGGUGucggaaucGUCCUCa -3' miRNA: 3'- uaCG-----AGCGUCGuuAGCCACu-------CAGGGG- -5' |
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| 18018 | 3' | -55.9 | NC_004665.1 | + | 33781 | 0.68 | 0.42503 |
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Target: 5'- cAUGUUUGCAGCGugcUCGaagauggcugggcaGUG-GUCCCCa -3' miRNA: 3'- -UACGAGCGUCGUu--AGC--------------CACuCAGGGG- -5' |
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| 18018 | 3' | -55.9 | NC_004665.1 | + | 10274 | 0.69 | 0.380301 |
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Target: 5'- uGUGCUC-CAGCGGUgaagaacuccuUGGUGGcGUCCCg -3' miRNA: 3'- -UACGAGcGUCGUUA-----------GCCACU-CAGGGg -5' |
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| 18018 | 3' | -55.9 | NC_004665.1 | + | 1391 | 0.69 | 0.344795 |
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Target: 5'- cAUGCUUGCGGCGAaccUUGGUG-GUCUg- -3' miRNA: 3'- -UACGAGCGUCGUU---AGCCACuCAGGgg -5' |
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| 18018 | 3' | -55.9 | NC_004665.1 | + | 35478 | 0.74 | 0.177206 |
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Target: 5'- -aGCUCGCGGUAGagggucaucUCGGUcugagGGGUUCCCa -3' miRNA: 3'- uaCGAGCGUCGUU---------AGCCA-----CUCAGGGG- -5' |
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| 18018 | 3' | -55.9 | NC_004665.1 | + | 21136 | 1.1 | 0.000413 |
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Target: 5'- cAUGCUCGCAGCAAUCGGUGAGUCCCCu -3' miRNA: 3'- -UACGAGCGUCGUUAGCCACUCAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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