Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18019 | 5' | -54.5 | NC_004665.1 | + | 2785 | 0.66 | 0.685292 |
Target: 5'- uGGAGCagGUUCACagcaCGACCACCacgcUCAUCa -3' miRNA: 3'- uCUUCG--CAGGUG----GCUGGUGGa---AGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 4353 | 0.66 | 0.67408 |
Target: 5'- -cAAGCGcUCGCCaaGAUCGCC-UCGCCa -3' miRNA: 3'- ucUUCGCaGGUGG--CUGGUGGaAGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 21551 | 0.66 | 0.67408 |
Target: 5'- uGAAGCGUCga--GAa-GCCUUCGCCa -3' miRNA: 3'- uCUUCGCAGguggCUggUGGAAGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 9225 | 0.66 | 0.662826 |
Target: 5'- cGAGGCGaUCCAgaaguUCGACUacaACCUcaaccaguUCGCCa -3' miRNA: 3'- uCUUCGC-AGGU-----GGCUGG---UGGA--------AGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 13563 | 0.66 | 0.662826 |
Target: 5'- cGAAcCGUgCAUCGcACCGUCUUCACCc -3' miRNA: 3'- uCUUcGCAgGUGGC-UGGUGGAAGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 7547 | 0.66 | 0.651541 |
Target: 5'- gAGcAGCGgcacgaCCGCCGuucucCCACgCUUCGCUg -3' miRNA: 3'- -UCuUCGCa-----GGUGGCu----GGUG-GAAGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 12192 | 0.66 | 0.628927 |
Target: 5'- uGAGGUGcgcuaCCACCGcUCGCCUgaguaCACCg -3' miRNA: 3'- uCUUCGCa----GGUGGCuGGUGGAa----GUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 28626 | 0.66 | 0.628927 |
Target: 5'- cAGcAGCcgcaCCACCGAUCACag-CACCa -3' miRNA: 3'- -UCuUCGca--GGUGGCUGGUGgaaGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 2635 | 0.67 | 0.617619 |
Target: 5'- gGGAAGCccauguGUCCgcccuGCCGAUUGCCUUCGa- -3' miRNA: 3'- -UCUUCG------CAGG-----UGGCUGGUGGAAGUgg -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 11911 | 0.68 | 0.539376 |
Target: 5'- --cAGCGacuugagaaCAUCGACCGCCUgcUCGCCu -3' miRNA: 3'- ucuUCGCag-------GUGGCUGGUGGA--AGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 23102 | 0.68 | 0.517579 |
Target: 5'- cGcAGC-UCCACgGGCCuCCUUCACg -3' miRNA: 3'- uCuUCGcAGGUGgCUGGuGGAAGUGg -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 7376 | 0.68 | 0.506813 |
Target: 5'- cGuGGCGUCUacaaggucucGCUGACCAuuCCUUCGCg -3' miRNA: 3'- uCuUCGCAGG----------UGGCUGGU--GGAAGUGg -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 14008 | 0.69 | 0.46477 |
Target: 5'- aGGguGCGaUUGCCGACCAgCUcaUCACCg -3' miRNA: 3'- -UCuuCGCaGGUGGCUGGUgGA--AGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 17919 | 0.7 | 0.424618 |
Target: 5'- uGGGAGUG-CCGCauaGGCCACCUugucgagcgUCACg -3' miRNA: 3'- -UCUUCGCaGGUGg--CUGGUGGA---------AGUGg -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 34733 | 0.71 | 0.350914 |
Target: 5'- gGGAGGgGUCUACCG-UCGCUUgugacgCACCg -3' miRNA: 3'- -UCUUCgCAGGUGGCuGGUGGAa-----GUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 29316 | 0.74 | 0.251575 |
Target: 5'- cGAAGCGUCCAUUGgaGCCACuCUcggucugCACCg -3' miRNA: 3'- uCUUCGCAGGUGGC--UGGUG-GAa------GUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 7115 | 0.78 | 0.13699 |
Target: 5'- uGAGGuCGUCCAUgCGACCACCaagaUCACCc -3' miRNA: 3'- uCUUC-GCAGGUG-GCUGGUGGa---AGUGG- -5' |
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18019 | 5' | -54.5 | NC_004665.1 | + | 21803 | 1.13 | 0.000428 |
Target: 5'- cAGAAGCGUCCACCGACCACCUUCACCa -3' miRNA: 3'- -UCUUCGCAGGUGGCUGGUGGAAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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