Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18020 | 3' | -51.3 | NC_004665.1 | + | 36986 | 0.67 | 0.771232 |
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Target: 5'- uGGUcUCCA-UAGGAUGCCCuuuaGGGu -3' miRNA: 3'- uUCGcAGGUaAUUCUACGGGuug-CCC- -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 21933 | 0.67 | 0.760394 |
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Target: 5'- uGGGCGUCCcgguugaUGAGGUGaaCCAGCGGc -3' miRNA: 3'- -UUCGCAGGua-----AUUCUACg-GGUUGCCc -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 12997 | 0.68 | 0.704246 |
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Target: 5'- cAGCGUCCAUgcAGcagcGUGCuCCAuACGGa -3' miRNA: 3'- uUCGCAGGUAauUC----UACG-GGU-UGCCc -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 26380 | 0.68 | 0.669486 |
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Target: 5'- cGAGCGUCCccuuGGAUgGCCUuuUGGGu -3' miRNA: 3'- -UUCGCAGGuaauUCUA-CGGGuuGCCC- -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 25121 | 0.69 | 0.634365 |
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Target: 5'- cGAGUG-CCAUgAAGGgcaGCUCGGCGGGu -3' miRNA: 3'- -UUCGCaGGUAaUUCUa--CGGGUUGCCC- -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 31350 | 0.69 | 0.610919 |
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Target: 5'- cAGCGUCCAcUAAGcgGCCUucUGGa -3' miRNA: 3'- uUCGCAGGUaAUUCuaCGGGuuGCCc -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 10289 | 0.69 | 0.608578 |
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Target: 5'- aGAGCGUCCuugucguGUGCuCCAGCGGu -3' miRNA: 3'- -UUCGCAGGuaauuc-UACG-GGUUGCCc -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 16812 | 0.73 | 0.426527 |
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Target: 5'- uGGGCGUCCAgUGgcgcauggucuuuccAGAgGCCCcAGCGGGa -3' miRNA: 3'- -UUCGCAGGUaAU---------------UCUaCGGG-UUGCCC- -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 26582 | 0.76 | 0.288451 |
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Target: 5'- cAAGCGcUCAUUAcuGAcGCCCAGCGGGa -3' miRNA: 3'- -UUCGCaGGUAAUu-CUaCGGGUUGCCC- -5' |
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| 18020 | 3' | -51.3 | NC_004665.1 | + | 22204 | 1.08 | 0.001605 |
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Target: 5'- cAAGCGUCCAUUAAGAUGCCCAACGGGu -3' miRNA: 3'- -UUCGCAGGUAAUUCUACGGGUUGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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