Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18023 | 3' | -55.5 | NC_004665.1 | + | 36453 | 0.66 | 0.577475 |
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Target: 5'- gGAGGaCGCCCUgAuGGGUCAcgaccGGCuGCu -3' miRNA: 3'- gUUCUcGCGGGAgUuCCCAGU-----UCG-CG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 1482 | 0.67 | 0.521934 |
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Target: 5'- ---uGGUGCCCUUGAGGaagAAGCGCg -3' miRNA: 3'- guucUCGCGGGAGUUCCcagUUCGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 4698 | 0.67 | 0.51106 |
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Target: 5'- uCGAGuGGCuGUCCcgUGAGGG-CAAGCGCu -3' miRNA: 3'- -GUUC-UCG-CGGGa-GUUCCCaGUUCGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 9131 | 0.67 | 0.500283 |
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Target: 5'- --cGAGCGCUCUgGAaacugucuccGGGUCAcugcccGCGCa -3' miRNA: 3'- guuCUCGCGGGAgUU----------CCCAGUu-----CGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 31645 | 0.68 | 0.42799 |
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Target: 5'- uGAGGGUGCCauagUGAGGGguuuuagCGGGUGCg -3' miRNA: 3'- gUUCUCGCGGga--GUUCCCa------GUUCGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 6546 | 0.69 | 0.412344 |
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Target: 5'- --cGAGa-CCCUCAAGGGcCAgauacucguuggcuuGGCGCa -3' miRNA: 3'- guuCUCgcGGGAGUUCCCaGU---------------UCGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 25477 | 0.69 | 0.371198 |
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Target: 5'- ---cGGCGCUgCUCAGGGGUCAGcuuGCGg -3' miRNA: 3'- guucUCGCGG-GAGUUCCCAGUU---CGCg -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 17022 | 0.71 | 0.303853 |
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Target: 5'- cCAAGAGCG-UCUCGAaGGUCGcuGGCGUa -3' miRNA: 3'- -GUUCUCGCgGGAGUUcCCAGU--UCGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 6481 | 0.73 | 0.221075 |
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Target: 5'- cCAAcGAGUaucugGCCCUUGAGGGUCucGGUGCu -3' miRNA: 3'- -GUU-CUCG-----CGGGAGUUCCCAGu-UCGCG- -5' |
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| 18023 | 3' | -55.5 | NC_004665.1 | + | 25010 | 1.12 | 0.000303 |
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Target: 5'- cCAAGAGCGCCCUCAAGGGUCAAGCGCa -3' miRNA: 3'- -GUUCUCGCGGGAGUUCCCAGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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