miRNA display CGI


Results 1 - 13 of 13 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18024 5' -55.6 NC_004665.1 + 37243 0.66 0.600487
Target:  5'- aACCGAGGCGAUCucacucagaGGUcaccggggaGAGGCUccUAUGg -3'
miRNA:   3'- gUGGUUCCGCUGG---------CCG---------UUCCGA--AUGC- -5'
18024 5' -55.6 NC_004665.1 + 137 0.66 0.600487
Target:  5'- aACCGAGGCGAUCucacucagaGGUcaccggggaGAGGCUccUAUGg -3'
miRNA:   3'- gUGGUUCCGCUGG---------CCG---------UUCCGA--AUGC- -5'
18024 5' -55.6 NC_004665.1 + 35271 0.66 0.600487
Target:  5'- uCACagAGGGCGAgUGGUcAGGCUUGg- -3'
miRNA:   3'- -GUGg-UUCCGCUgGCCGuUCCGAAUgc -5'
18024 5' -55.6 NC_004665.1 + 28990 0.66 0.577967
Target:  5'- aCGCCAGcuuGGCGAgCaGCcucacGGGCUUGCa -3'
miRNA:   3'- -GUGGUU---CCGCUgGcCGu----UCCGAAUGc -5'
18024 5' -55.6 NC_004665.1 + 13763 0.66 0.552307
Target:  5'- aCACCGugcucaacGGUGACauccacugggucaaCGGCAAGGCUgcugGCa -3'
miRNA:   3'- -GUGGUu-------CCGCUG--------------GCCGUUCCGAa---UGc -5'
18024 5' -55.6 NC_004665.1 + 16502 0.67 0.533564
Target:  5'- uGCCAAGGacACCGGCAuGGGCa---- -3'
miRNA:   3'- gUGGUUCCgcUGGCCGU-UCCGaaugc -5'
18024 5' -55.6 NC_004665.1 + 16211 0.67 0.533564
Target:  5'- uGCCAGuGGCGAUCaGGUacGAGGCguugagGCGu -3'
miRNA:   3'- gUGGUU-CCGCUGG-CCG--UUCCGaa----UGC- -5'
18024 5' -55.6 NC_004665.1 + 5173 0.67 0.511811
Target:  5'- aACCGAGGgucgcucaaUGACCGGCAuGGUUUcCa -3'
miRNA:   3'- gUGGUUCC---------GCUGGCCGUuCCGAAuGc -5'
18024 5' -55.6 NC_004665.1 + 4416 0.67 0.490434
Target:  5'- uGgCGAGGCGAUCuuGGCGAGcGCUUGg- -3'
miRNA:   3'- gUgGUUCCGCUGG--CCGUUC-CGAAUgc -5'
18024 5' -55.6 NC_004665.1 + 3294 0.67 0.479902
Target:  5'- aGCCAucAGGCgugacccaauaGACCGGCAuugcAGGCUU-Ca -3'
miRNA:   3'- gUGGU--UCCG-----------CUGGCCGU----UCCGAAuGc -5'
18024 5' -55.6 NC_004665.1 + 2712 0.71 0.289846
Target:  5'- aACCGucgaAGGCaAUCGGCAGGGCggACa -3'
miRNA:   3'- gUGGU----UCCGcUGGCCGUUCCGaaUGc -5'
18024 5' -55.6 NC_004665.1 + 2195 0.73 0.216414
Target:  5'- uCACCc-GGCuuGAUCGGCAGGGCUUcACGu -3'
miRNA:   3'- -GUGGuuCCG--CUGGCCGUUCCGAA-UGC- -5'
18024 5' -55.6 NC_004665.1 + 27593 1.09 0.00053
Target:  5'- uCACCAAGGCGACCGGCAAGGCUUACGg -3'
miRNA:   3'- -GUGGUUCCGCUGGCCGUUCCGAAUGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.