Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18027 | 5' | -47.6 | NC_004665.1 | + | 20616 | 0.66 | 0.94002 |
Target: 5'- -gAUCAccAGCAGGgacguaguucuUGGUCAGGcGAGCACg -3' miRNA: 3'- ugUAGU--UCGUUC-----------GCUAGUUC-CUUGUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 12258 | 0.67 | 0.907978 |
Target: 5'- uAC-UCAGGCGAGCGGU---GGuAGCGCa -3' miRNA: 3'- -UGuAGUUCGUUCGCUAguuCC-UUGUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 4436 | 0.67 | 0.90579 |
Target: 5'- cACGUCGgucacagagcuucuGGCgAGGCGAUCuuGGcGAGCGCu -3' miRNA: 3'- -UGUAGU--------------UCG-UUCGCUAGu-UC-CUUGUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 4155 | 0.67 | 0.903572 |
Target: 5'- --uUCAGGUAgcggaugguuuguugGGCGAugUCGAGGGACAg -3' miRNA: 3'- uguAGUUCGU---------------UCGCU--AGUUCCUUGUg -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 29766 | 0.68 | 0.867719 |
Target: 5'- uCAUCAAGgAAGCaAUCGagaAGGAAgGCa -3' miRNA: 3'- uGUAGUUCgUUCGcUAGU---UCCUUgUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 2031 | 0.68 | 0.865057 |
Target: 5'- gACAUCGcGguAGCGGUUGAgcgcucgcuuugucGGGACACg -3' miRNA: 3'- -UGUAGUuCguUCGCUAGUU--------------CCUUGUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 31811 | 0.69 | 0.849475 |
Target: 5'- cACGUCGAGUugcacCGAUCAAGGugacCACa -3' miRNA: 3'- -UGUAGUUCGuuc--GCUAGUUCCuu--GUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 26420 | 0.7 | 0.788484 |
Target: 5'- gACAUCGGuGCGGGCGAguggaAAGGcguaGACGCa -3' miRNA: 3'- -UGUAGUU-CGUUCGCUag---UUCC----UUGUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 11838 | 0.71 | 0.759665 |
Target: 5'- aGCAaggCGAGCAGGCGGUCGAuguucucaagucgcuGGGcuACACc -3' miRNA: 3'- -UGUa--GUUCGUUCGCUAGUU---------------CCU--UGUG- -5' |
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18027 | 5' | -47.6 | NC_004665.1 | + | 30742 | 1.12 | 0.002181 |
Target: 5'- gACAUCAAGCAAGCGAUCAAGGAACACa -3' miRNA: 3'- -UGUAGUUCGUUCGCUAGUUCCUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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