Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18028 | 3' | -47.9 | NC_004665.1 | + | 12761 | 0.66 | 0.931838 |
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Target: 5'- --cGCCAGCucGAAgcggACGGUGGCgUCu -3' miRNA: 3'- uuaCGGUUGc-CUUaa--UGCCACUGaAG- -5' |
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| 18028 | 3' | -47.9 | NC_004665.1 | + | 6389 | 0.67 | 0.912265 |
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Target: 5'- aGAUGCCAGCGaggugacuGAggUGCGGUGuCgUCc -3' miRNA: 3'- -UUACGGUUGC--------CUuaAUGCCACuGaAG- -5' |
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| 18028 | 3' | -47.9 | NC_004665.1 | + | 35888 | 0.7 | 0.784595 |
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Target: 5'- --gGCCAACGGAuucGUaGCGGUGGaagcggUCa -3' miRNA: 3'- uuaCGGUUGCCU---UAaUGCCACUga----AG- -5' |
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| 18028 | 3' | -47.9 | NC_004665.1 | + | 7347 | 0.7 | 0.784595 |
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Target: 5'- cGGUGCCGACuggagugaacaGGAAUUGCuuGGUGgACUUUg -3' miRNA: 3'- -UUACGGUUG-----------CCUUAAUG--CCAC-UGAAG- -5' |
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| 18028 | 3' | -47.9 | NC_004665.1 | + | 36892 | 0.72 | 0.656664 |
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Target: 5'- --gGCCucugGCGGA--UGCGGUGACUg- -3' miRNA: 3'- uuaCGGu---UGCCUuaAUGCCACUGAag -5' |
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| 18028 | 3' | -47.9 | NC_004665.1 | + | 10057 | 0.72 | 0.644576 |
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Target: 5'- cGAUGCaguCGGcGUUGCGG-GACUUCu -3' miRNA: 3'- -UUACGguuGCCuUAAUGCCaCUGAAG- -5' |
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| 18028 | 3' | -47.9 | NC_004665.1 | + | 32724 | 1.08 | 0.003599 |
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Target: 5'- cAAUGCCAACGGAAUUACGGUGACUUCu -3' miRNA: 3'- -UUACGGUUGCCUUAAUGCCACUGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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