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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18029 | 3' | -50.4 | NC_004665.1 | + | 20962 | 0.67 | 0.796147 |
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Target: 5'- ----cGGCGGUCuGCguccUGaaGCUGCUGGu -3' miRNA: 3'- cguaaCCGUCAG-CGaa--AC--UGACGACC- -5' |
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| 18029 | 3' | -50.4 | NC_004665.1 | + | 25001 | 0.69 | 0.708114 |
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Target: 5'- cGCggUGGUAGUUGUcagUGauggacaugcGCUGCUGGc -3' miRNA: 3'- -CGuaACCGUCAGCGaa-AC----------UGACGACC- -5' |
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| 18029 | 3' | -50.4 | NC_004665.1 | + | 19783 | 0.7 | 0.661606 |
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Target: 5'- ----gGGCAGcCGCUggGGaUGCUGGg -3' miRNA: 3'- cguaaCCGUCaGCGAaaCUgACGACC- -5' |
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| 18029 | 3' | -50.4 | NC_004665.1 | + | 1311 | 0.71 | 0.579468 |
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Target: 5'- aGCAgcGGUu-UCGCUacUUGGCUGUUGGa -3' miRNA: 3'- -CGUaaCCGucAGCGA--AACUGACGACC- -5' |
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| 18029 | 3' | -50.4 | NC_004665.1 | + | 26348 | 0.87 | 0.066925 |
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Target: 5'- --cUUGGCGGUCGCUUggGAUUGCUGGc -3' miRNA: 3'- cguAACCGUCAGCGAAa-CUGACGACC- -5' |
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| 18029 | 3' | -50.4 | NC_004665.1 | + | 34386 | 1.14 | 0.000895 |
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Target: 5'- cGCAUUGGCAGUCGCUUUGACUGCUGGu -3' miRNA: 3'- -CGUAACCGUCAGCGAAACUGACGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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